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Status |
Public on Feb 28, 2018 |
Title |
Day4 microglia bio replicate 7 |
Sample type |
RNA |
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Source name |
microglia from day 4 post-infection 6-week old mouse brains
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Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 strain: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (Jackson) age: 6 weeks infection status: day 4 post-MHV infection time point: day 4 post-MHV-infection tissue: brain cell type: FACS-sorted microglia
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated ds -cDNA were prepared according to the standard Affymetrix protocol from 1 ng total RNA (3' IVT Pico Reagent Kit, Affymetrix).
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Hybridization protocol |
Following fragmentation, ds-cDNA were hybridized for 18 hr at 45C with 60 rpm rotation on Affymatrix mouse Clariom S array.
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Scan protocol |
Clariom S array was scanned using Affymatrix Model 3000 scanner.
|
Data processing |
Partek Genomics Suite was used to analyze the data. RMA analysis was used. Anova-generated p-values were corrected using false-discovery rate. Genes with adjusted p-values <0.05 and a fold-change >2 was considered significant. affymetrix-algorithm-name = sst-rma-gene-full affymetrix-algorithm-version = 1.0 program-name = Expression Console program-version = 1.4.1.46
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Submission date |
Sep 01, 2017 |
Last update date |
Feb 28, 2018 |
Contact name |
Dorthea Wheeler |
E-mail(s) |
dorthea-wheeler@uiowa.edu
|
Organization name |
University of Iowa
|
Street address |
51 Newton Road
|
City |
iowa City |
State/province |
IA |
ZIP/Postal code |
52242 |
Country |
USA |
|
|
Platform ID |
GPL23038 |
Series (2) |
GSE103380 |
Gene expression of microglia from naïve or MHV infected mouse brains |
GSE103727 |
Gene expression of macrophages and microglia from naïve or MHV infected mouse brains |
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