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Sample GSM277456 Query DataSets for GSM277456
Status Public on Mar 27, 2008
Title cumulus_PCOS_Obese_rep3
Sample type RNA
 
Source name luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep3
Organism Homo sapiens
Characteristics Gender: Female, Age: 37, BMI: 31, Tissue: luteinized cumulus cells
Treatment protocol Patients were treated with gonadotropins
Growth protocol Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
Description All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
Data processing Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
 
Submission date Mar 26, 2008
Last update date Aug 28, 2018
Contact name Shlomit Kenigsberg
E-mail(s) shlomit.kenig@gmail.com
Organization name Create Fertility Centre
Department Research
Lab Ovarian Biology Research
Street address 790 Bay st.
City Toronto
State/province Ontario
ZIP/Postal code M4N1S8
Country Canada
 
Platform ID GPL570
Series (1)
GSE10946 Gene expression microarray profiles of cumulus cells in lean and overweight-obese polycystic ovary syndrome patients
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA signal, in log2 scale.

Data table
ID_REF VALUE
1007_s_at 9.225881753
1053_at 5.071355082
117_at 4.310755516
121_at 7.828114508
1255_g_at 4.156395832
1294_at 6.388299482
1316_at 4.869295358
1320_at 4.538779076
1405_i_at 2.831958151
1431_at 3.206617615
1438_at 5.086523888
1487_at 6.383046942
1494_f_at 5.938743814
1552256_a_at 12.47233096
1552257_a_at 7.622428929
1552258_at 4.25492454
1552261_at 4.833729906
1552263_at 5.119850219
1552264_a_at 5.330452651
1552266_at 4.153359755

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM277456.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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