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Sample GSM2775077 Query DataSets for GSM2775077
Status Public on Sep 06, 2019
Title ZT0_ALDH_p
Sample type RNA
 
Source name ZT0, ALDH(+)
Organism Mus musculus
Characteristics strain: BALB/C
tissue: Tumor
Treatment protocol Non treatmment
Growth protocol 4T1 cell was impranted in mice
Extracted molecule total RNA
Extraction protocol The total RNA was isolated from cells using ReliaPrep TM RNA Cell Miniprep System (Promega) according to the manufacturer's instructions.
Label Cy3
Label protocol cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
 
Hybridization protocol cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Mouse Gene Expression Microarray 8x60K v2 ; Agilent Technologies) according to the manufacturer's instructions.
Scan protocol The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
Data processing The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. The raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
 
Submission date Sep 07, 2017
Last update date Sep 06, 2019
Contact name naoya matsunaga
E-mail(s) matunaga@phar.kyushu-u.ac.jp
Organization name kyushu university
Street address maidashi 3-1-1, higashi-ku
City fukuoka
ZIP/Postal code 8128582
Country Japan
 
Platform ID GPL21163
Series (1)
GSE103597 ALDH high activity or low activity cell in 4T1 bearing mice

Data table header descriptions
ID_REF
VALUE quantile normalized signal (non-log scaled) and ABS CALL.
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
A_30_P01017428 39.3157 P
A_30_P01017437 203.73355 P
A_30_P01017440 3.82363425 A
A_30_P01017441 9.7858915 A
A_30_P01017444 106.086405 P
A_30_P01017445 34.53679 P
A_30_P01017447 104.0466175 P
A_30_P01017448 125.4708675 P
A_30_P01017453 2.887434 A
A_30_P01017454 23.1437925 P
A_30_P01017455 33.9620575 P
A_30_P01017466 3.51713325 A
A_30_P01017468 25.7905075 P
A_30_P01017473 48.21456 P
A_30_P01017482 15293.37745 P
A_30_P01017490 340.1284 P
A_30_P01017495 29.0789925 P
A_30_P01017497 3.48144575 A
A_30_P01017503 3.95835175 A
A_30_P01017505 69.9130175 P

Total number of rows: 56605

Table truncated, full table size 1434 Kbytes.




Supplementary file Size Download File type/resource
GSM2775077_US11030397_257480910068_S01_GE1_107_Sep09_1_2.txt.gz 12.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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