|
| Status |
Public on Dec 22, 2017 |
| Title |
Endothelial cells at day2, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Endothelial cells at day2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Tibialis anterior muscle
time point: Day 8 post-injury
cell type: Endothelial cells
|
| Treatment protocol |
Endothelial cells (ECs) and Satellite cells (SCs) were isolated by FACS as CD45-Sca1+CD34+CD31+ and as CD45-Sca1-CD34+/loCD31-alpha7integrin+, respectively.
|
| Growth protocol |
Tibialis Anterior mouse muscle was injured with cardiotoxin and muscles were recovered before (day0) and days 2, 4, 8 post-injury. Muscles were minced, enzymatically digested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 750 pg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3,6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip MouseGene2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GC3000 Scanner.
|
| Description |
12_EC2-5_(MoGene-2_0-st).CEL
|
| Data processing |
The data were normalized with RMA.
|
| |
|
| Submission date |
Sep 11, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Benedicte Chazaud |
| E-mail(s) |
benedicte.chazaud@inserm.fr
|
| Organization name |
Université Claude Bernard Lyon 1
|
| Department |
Institut NeuroMyoGène
|
| Street address |
8 Abenue Rockefeller
|
| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL18802 |
| Series (1) |
| GSE103684 |
Expression data from Satellite cells and Endothelial cells isolated at different time points during skeletal muscle regeneration |
|
