|
Status |
Public on Feb 14, 2018 |
Title |
OB_WT_18M_R1 |
Sample type |
RNA |
|
|
Source name |
Olfactory bulb
|
Organism |
Mus musculus |
Characteristics |
gender: female genotype/variation: wild type tissue: Olfactory bulb
|
Extracted molecule |
total RNA |
Extraction protocol |
For olfactory bulb mRNA extraction, the Maxwell 16 simplyRNA kit (Promega) was used
|
Label |
biotin
|
Label protocol |
The sense cDNA was prepared from 1 ng of total RNA using the Affymetrix GeneChip® WT Pico Kit (PN902623). The sense strand cDNA was then fragmented and biotinylated according to the manufacturer's instructions.
|
|
|
Hybridization protocol |
Labeled sense cDNA was hybridized to the GeneChip® Mouse Clariom S Arrays, washed and stained on a GeneChip® Fluidics 450 workstation (Affymetrix) according to the manufacturer protocols.
|
Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000 7G
|
Data processing |
Data were normalized with Bioconductor software using RMA algorithm.
|
|
|
Submission date |
Sep 13, 2017 |
Last update date |
Feb 14, 2018 |
Contact name |
Victor Segura |
E-mail(s) |
vsegura@unav.es
|
Organization name |
FIMA
|
Department |
Unit of Proteomics, Genomics and Bioinformatics
|
Street address |
Avda. Pío XII, 55
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL23038 |
Series (1) |
GSE103835 |
The olfactory molecular snapshot in aged Tg2576 Alzheimer´s disease mouse model |
|