|
| Status |
Public on Sep 14, 2018 |
| Title |
Kras spleen NK, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Kras spleen NK
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Kras
age: 6-8 weeks
infected with: Lenti-Cre for induction of lung cancer
time point: Stage 3 (16 weeks after Lenti-Cre delivery)
tissue: spleen
cell type: Natural killer (NK)
|
| Treatment protocol |
NK cells were isolated from spleens and lungs of WT and Stage 3 Kras mice, and we lysed the cells in TRIzol (Invitrogen) and freezed them in refrigerator (-80°C).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using miRNeasy Mini Kit and RNase-Free Dnase Set.Total RNA quality was assessed with a Pico Chip on an Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
indocarbocyanine
|
| Label protocol |
RNA was amplified and abeling of CTP with indocarbocyanine was done with Agilent one-color labeling kits (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Indocarbocyanine-labeled cDNA was assessed with the NanoDrop ND-2000 (Thermo Scientific), and equal amounts of indocarbocyanine-labeled target were hybridized to Agilent SurePrint G3 Mouse Gene Expression (8x60K) microarrays（Design ID：74809）.
|
| Scan protocol |
Arrays were scanned with the Agilent Scanner G2505C(Agilent Technologies).
|
| Description |
SAMPLE 6
|
| Data processing |
Raw signal intensities were extracted and quantile normalized with Feature Extraction v10.7.1.1 software(Agilent Technologies).
|
| |
|
| Submission date |
Sep 14, 2017 |
| Last update date |
Sep 14, 2018 |
| Contact name |
Shu Zhu |
| E-mail(s) |
zhushu@ustc.edu.cn
|
| Organization name |
University of Science and Technology of China
|
| Street address |
443 Huangshan Road, Hefei, Anhui, China
|
| City |
Hefei |
| ZIP/Postal code |
230027 |
| Country |
China |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE103856 |
Expression data from lung and spleen natural killer cells of WT and Kras mice |
|
