|
| Status |
Public on May 01, 2018 |
| Title |
BLIMN_I693_CD206- |
| Sample type |
RNA |
| |
|
| Source name |
Primary pancreatic FACS-sorted cells from immunized BL001 treated mice
|
| Organism |
Mus musculus |
| Characteristics |
age: 4 month old
tissue: Pancreatic macrophages
treatment: pre-proinsulin and BL001
|
| Treatment protocol |
Animals were maintained under standard conditions, immunized with pre-proinsulin and BL001- or Vehicle-treated for 8 weeks
|
| Growth protocol |
Standard feeding
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from FACS-sorted cells, obtained from different pool of mice per group, in triplicates was isolated using the RNAesay micro kit according to the manufacturer´s protocol (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 1 ng total RNA Labeling Protocol (The Ambion WT Expression kit for Affymetrix 3’-IVT Pico, Life Technologies Corporation, 2009).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of double-stranded cDNA were hybridized for 14 hr at 40C on Clariom™ S Assay, Mouse. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
|
| Scan protocol |
Microarrays were scanned using GeneChip® Scanner 3000 7G
|
| Description |
The mouse presented healthy appearance
|
| Data processing |
The .CEL files were generated using Affymetrix GCOS software and further processed into expression values using Robust Multiarray Average (RMA) method by Irrizarry et al. The following statistical data analysis were performed using the limma package of R/Bioconductor project.
|
| |
|
| Submission date |
Sep 27, 2017 |
| Last update date |
May 01, 2018 |
| Contact name |
Benoit Raymond Gauthier |
| E-mail(s) |
benoit.gauthier@cabimer.es
|
| Organization name |
Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER)
|
| Department |
Stem Cells
|
| Lab |
Pancreatic Islet Development and Regeneration Unit
|
| Street address |
Americo Vespucio Avenue
|
| City |
Seville |
| State/province |
Seville |
| ZIP/Postal code |
41092 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE104322 |
LRH-1/NR5A2 induces M1 to M2 macrophage phenotypic switch |
| GSE104328 |
LRH-1/NR5A2 for the treatment of autoimmune diseases |
|
