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Sample GSM280203 Query DataSets for GSM280203
Status Public on Oct 02, 2008
Title pUC-18_EBER2-L2_2
Sample type RNA
 
Source name HEK293 cell line
Organism Homo sapiens
Characteristics pUC-18-EBER2-L2 transfected HEK293 cells
Extracted molecule total RNA
Extraction protocol RNA was extracted from the cell lines using RNeasy Midi Kit (Quiagen) according to the instructions provided by the manufacturer
Label DIG-UTP
Label protocol Digoxigenin-UTP labeled cRNA was generated and amplified from 2 μg of total RNA from each sample using Applied Biosystems NanoAmp RT-IVT Labeling Kit (P/N 4365715) according to the manufacturer’s protocol(P/N 4365710).
 
Hybridization protocol Array hybridization was performed for 16 hrs at 55 oC. Pre-Hybridization, Hybridization, antibody binding and Chemiluminescence reaction were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) following the manufacturer’s protocol (P/N 4339627).
Scan protocol image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer (P/N 4338036) following the manufacturer’s protocol (P/N 4339629).
Description pUC-18-EBER2-L2 transfected HEK293 cells
Data processing Images were auto-gridded and the chemiluminescent signals were quantified, corrected for background, and finally, spot- and spatially-normalized using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer software v 1.1 (P/N 4336391). Median normalization and Probabilty for a gene to be expressed(A) were calculated with proprietary software.
 
Submission date Apr 08, 2008
Last update date Oct 01, 2008
Contact name Arndt Benecke
E-mail(s) arndt@ihes.fr
Phone +33160926665
Fax +33160926609
URL http://seg.ihes.fr
Organization name Institut des Hautes Etudes Scientifiques & I.R.I.-CNRS
Department Integrative Biology
Lab Systems Epigenomics
Street address 35 route des Chartres
City Bures-sur-Yvette
ZIP/Postal code 91440
Country France
 
Platform ID GPL2986
Series (1)
GSE11093 EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr Virus infection.

Data table header descriptions
ID_REF
VALUE Mediane Normalized Signal
VAR Coefficient of Variation
A Probability for a gene to be expressed

Data table
ID_REF VALUE VAR A
100002 0.3806 0.6100 0.1628
100027 0.6710 0.2700 0.2648
100036 0.3419 2.5200 0.1805
100037 0.8839 0.9500 0.3095
100039 0.6000 0.4100 0.2405
100044 0.3419 1.3500 0.1508
100045 1.4903 1.3400 0.4810
100051 0.3032 1.3000 0.1377
100052 0.3484 2.4400 0.1793
100057 1.0839 0.8600 0.3670
100058 212.6839 0.0400 1.0000
100060 0.6645 1.2000 0.2488
100062 2.4452 0.9400 0.6301
100064 0.4194 11.2400 0.4031
100079 0.8065 0.2200 0.2746
100089 16.2387 0.0900 1.0000
100093 12.5677 0.0500 1.0000
100095 0.8903 248.9500 0.4955
100100 66.4645 0.0400 1.0000
100102 0.4581 0.7300 0.1798

Total number of rows: 32576

Table truncated, full table size 900 Kbytes.




Supplementary file Size Download File type/resource
GSM280203.txt.gz 354.2 Kb (ftp)(http) TXT
Processed data included within Sample table

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