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Sample GSM280422 Query DataSets for GSM280422
Status Public on Mar 01, 2009
Title Tibialis anterior muscle from MLC-SOCS-3/C57Bl6J mice rep2
Sample type RNA
 
Source name Tibialis anterior muscle
Organism Mus musculus
Characteristics C57Bl6J mice
transgenic animals expressing constitutively SOCS-3 specifically in skeletal muscle (under the control of MLC-1 promoter)
Treatment protocol Tibialis muscle was extracted from 6h starved mice and quickly frozen in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA.
 
Hybridization protocol Following fragmentation and labeling, 3ug of DNA were hybridized for 17 hr at 45C on Mouse gene 1.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from tibialis muscle extracted from 11 week-old mouse
Data processing The data were analyzed with Expression Console using Affymetrix default analysis settings and Gene Level-RMA sketch as normalization method.
 
Submission date Apr 09, 2008
Last update date Nov 21, 2008
Contact name Kevin Lebrigand
Organization name IPMC/CNRS
Lab Functional Genomics Platform of Nice-Sophia-Antipolis, France.
Street address 660 route des lucioles
City Valbonne - Sophia-Antipolis
ZIP/Postal code 06560
Country France
 
Platform ID GPL6246
Series (1)
GSE11105 Expression data from tibialis anterior muscle

Data table header descriptions
ID_REF
VALUE RMA sketch Signal intensity

Data table
ID_REF VALUE
10344614 6.672349
10344618 2.34012
10344620 3.6708
10344622 7.317395
10344624 11.03011
10344633 10.032
10344637 8.363558
10344653 3.759044
10344658 9.514819
10344674 2.97692
10344679 4.534233
10344707 10.31956
10344713 9.590124
10344717 3.40478
10344719 6.08002
10344723 8.211646
10344725 10.01692
10344741 10.57657
10344743 3.916924
10344750 8.120733

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM280422.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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