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Sample GSM280431 Query DataSets for GSM280431
Status Public on Apr 09, 2008
Title Danio_Brain_Starved_3 (X2BS3)
Sample type RNA
Source name Brain
Organism Danio rerio
Characteristics Strain: Scientific Hatcheries, Sex: Female, N: 3 fish, Birthdate: 8172005, Start date: 432006, Mean Mass: 298.9, Mean Length: 24.3, Mean SGR: -0.649, Mean GSI: 0.065
Treatment protocol Groups of eight females of the same age were randomly assigned to each of twelve 3-L tanks (10 cm wide x 15 cm tall x 25 cm deep) arranged on the same shelf of a flow-through rearing system. Test tanks were assigned to starved or control treatments in an alternating manner so that treatments were uniformly distributed across fish ages and tank location. Fish in the starved treatment received no food for 21 days, while fish in the control treatment continued to be fed to apparent satiety three times daily.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the TRIzol method (Invitrogen, Carlsbad, CA) following the manufacturer's protocol. After extraction, RNA from three fish was pooled for each tank for analysis.
Label Biotin
Label protocol For each array, up to 10 ug of total RNA were converted to cDNA. Biotinylated cRNA was then produced in vitro using the GeneChip Expression 3' amplification one-cycle target labeling kit (Affymetrix).
Hybridization protocol Microarrays were hybridized with fragmented biotinylated cRNA for 16 h at 45°C with constant rotation (45 r.p.m.), and processed using the Affymetrix GeneChip Fluidic Station 450. Streptavidin-conjugated phycoerythrin (SAPE) was used for staining, followed by amplification using a biotinylated anti-streptavidin antibody. This was followed by another round of SAPE prior to scanning using a GeneChip Scanner 3000 (Affymetrix).
Scan protocol Scanned using a GeneChip Scanner 3000 (Affymetrix).
Description X2BS3
Data processing All analyses of microarray data were conducted using Bioconductor for R ( Data for brain and liver were normalized and analyzed separately because variances of expression differed considerably between the tissues. The microarray data were filtered as described by Robison et al. (2008). Filtered probesets were then normalized using Robust Multiarray Average (RMA).
Submission date Apr 09, 2008
Last update date Apr 09, 2008
Contact name Robert Edward Drew
Phone 208-885-8478
Fax 208-885-7905
Organization name University of Idaho
Department Biological Sciences
Lab Robison
Street address Life Sciences South Rm 252
City Moscow
State/province ID
ZIP/Postal code 83844-3051
Country USA
Platform ID GPL1319
Series (1)
GSE11107 Effect of starvation on the transcriptomes of the brain and liver in adult female zebrafish (Danio rerio)

Data table header descriptions
VALUE RMA Normalized Log2 data

Data table
AFFX-BioB-3_at 6.53333105
AFFX-BioB-5_at 7.227911139
AFFX-BioB-M_at 6.952060121
AFFX-BioC-3_at 8.220729655
AFFX-BioC-5_at 8.089278363
AFFX-BioDn-3_at 10.58425628
AFFX-BioDn-5_at 9.62223756
AFFX-CreX-3_at 12.66370358
AFFX-CreX-5_at 11.63197458
AFFX-DapX-3_at 8.094284215
AFFX-DapX-5_at 5.985653168
AFFX-DapX-M_at 7.120383583
AFFX-Dr-AB076373-1_at 3.317059323
AFFX-Dr-acta1-3_at 8.405301258
AFFX-Dr-acta1-5_at 5.684736341
AFFX-Dr-acta1-5_x_at 6.244330129
AFFX-Dr-acta1-M_at 6.712387359
AFFX-Dr-AF292559-1_at 2.924221179
AFFX-Dr-AF292559-2_s_at 2.43728658
AFFX-Dr-AF292559-3_s_at 2.668134568

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.

Supplementary file Size Download File type/resource
GSM280431.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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