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Sample GSM2806962 Query DataSets for GSM2806962
Status Public on Dec 01, 2017
Title IFNg_C12-7_3
Sample type RNA
 
Source name IFNg_C12-7_3
Organism Mus musculus
Characteristics t-cell_clone: 12-7
phenotype: IFN-g
Growth protocol 12-7, 12-7, 13-11, 13-7, 16-8 maintained by weekly antigen restimulation with immune B cell APC; 3-10 weekly restimulation with immune splenocyte APC; all T cell clones in RPMI media with 3 ng/ml IL-7 for 48h prior to extraction
Extracted molecule total RNA
Extraction protocol RNEasy with G-eliminator (Qiagen)
Label biotin
Label protocol Affymetrix WT Plus using 100ng of total RNA
 
Hybridization protocol hybridized 17h using the standard Affymetrix protocol
Scan protocol Affymetrix GeneChip Command Console used to generate CEL files
Data processing Imported into Partek Genomics Suite 6.6.RMA was generated using RMA background correction, quantile normalization and summarization by Median Polis
Clariom_S_Mouse_HT.r1.pgf
Clariom_S_Mouse_HT.r1.mps
 
Submission date Oct 10, 2017
Last update date Jan 23, 2018
Contact name Raymond Morris Johnson
E-mail(s) raymond.johnson@yale.edu
Organization name Yale University
Department Med/Infectious Diseases
Street address PO Box 208022
City New Haven
State/province CT
ZIP/Postal code 06520-8022
Country USA
 
Platform ID GPL23038
Series (1)
GSE104743 B cell presentation of Chlamydia antigen selects out protective CD4ɣ13 T cells; implications for genital tract tissue resident memory lymphocyte clusters

Data table header descriptions
ID_REF
VALUE log base 2 RMA

Data table
ID_REF VALUE
TC0100000014.mm.2 10.23
TC0100000018.mm.2 4.91
TC0100000021.mm.2 9.14
TC0100000022.mm.2 3.80
TC0100000023.mm.2 3.59
TC0100000027.mm.2 10.88
TC0100000038.mm.2 9.34
TC0100000039.mm.2 5.65
TC0100000041.mm.2 5.19
TC0100000044.mm.2 9.02
TC0100000045.mm.2 4.94
TC0100000048.mm.2 8.52
TC0100000055.mm.2 4.42
TC0100000056.mm.2 4.61
TC0100000064.mm.2 4.50
TC0100000071.mm.2 4.02
TC0100000082.mm.2 4.36
TC0100000092.mm.2 10.39
TC0100000097.mm.2 7.20
TC0100000101.mm.2 7.97

Total number of rows: 22206

Table truncated, full table size 503 Kbytes.




Supplementary file Size Download File type/resource
GSM2806962_RO13M003_Clariom_S_Mouse_.CEL.gz 1.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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