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Sample GSM282130 Query DataSets for GSM282130
Status Public on Nov 24, 2008
Title S404AGR(Dex)::1
Sample type RNA
 
Source name S404A Glucocorticoid receptor alpha treated for 6 hr, replicate 1
Organism Homo sapiens
Characteristics Human osteosarcoma, female
Extracted molecule total RNA
Extraction protocol The RNeasy RNA extraction kit, with on column DNAse digestion (Qiagen) was used, according to manufacturer's protocol.
Label Cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
 
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description Cells were grown in DMEM-F12 medium, supplemented with 10% Fetal Bovine Serum. 48 hour before treatments, cells were placed in DMEM-F12 medium supplemented with 10% charcoal-dextran-treated FBS. Cells were not treated or were treated with 100 nM dexamethasone for 6 hr.
Data processing Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The Agilent Feature Extraction Software performed error modeling, adjusting for additive and multiplicative noise. The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
 
Submission date Apr 17, 2008
Last update date Jan 05, 2012
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL4133
Series (1)
GSE11205 Glycogen Synthase Kinase-3-beta Regulates Glucocorticoid Signaling by Phosphorylating the Glucocorticoid Receptor

Data table header descriptions
ID_REF Agilent Feature Number
VALUE Log2 intensity exported from Rosetta Resolver

Data table
ID_REF VALUE
44919 10.08872
8563 4.59951
28336 5.52051
5006 15.50101
28801 14.11325
29429 15.87044
38756 8.79917
31922 13.56512
3509 2.60657
27587 12.24762
18581 8.49912
10122 9.82259
14308 7.23917
35097 4.49846
22537 3.97911
1387 10.02737
41336 8.24981
33919 3.38948
24114 14.58471
9363 10.49652

Total number of rows: 40982

Table truncated, full table size 560 Kbytes.




Supplementary file Size Download File type/resource
GSM282130.txt.gz 8.8 Mb (ftp)(http) TXT
GSM282130_US22502625_251485022326_S01_H.tif.gz 61.1 Mb (ftp)(http) TIFF
GSM282130_US22502625_251485022326_S01_L.tif.gz 45.8 Mb (ftp)(http) TIFF
Processed data included within Sample table

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