|
| Status |
Public on Jan 17, 2018 |
| Title |
BV173 doxycycline 1 |
| Sample type |
RNA |
| |
|
| Source name |
cell line BV173
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BV173
|
| Treatment protocol |
Cells were plated at 250,000/mL with (DOX) or without (CTRL) doxycycline 1 mg/L for 24 hours
|
| Growth protocol |
Cells were expanded in Iscove's modified DMEM supplemented with 10% featl bovine serum, 100 U/mL penicicllin/streptomycin and 2 mM L-glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 4-5 millions of cells by using the RNeasy Plus Mini-Kit (Qiagen #74134)
|
| Label |
biotin
|
| Label protocol |
biotin labeled cDNA was sythesized using the Affymetrix WT Plus Kit (Thermo Fisher Scientific) for BV173 cells or the Ovation PICO WTA-system V2 and the FL-Ovation 8 cDNA biodin module (NuGen Technologie) for SUP-B15 cells
|
| |
|
| Hybridization protocol |
cDNA from BV173 cells was hybridized onto Human Clariom S Assay (Affymetrix) and cDNA from SUP-B15 was hybridized onto Human Gene 1.0 ST Array (Affymetrix) folowing the manifacturer instructions.
|
| Scan protocol |
Arrays were scanned by using the Affymetrix Gene Chip Scanner 3000 using the Command Console Software.
|
| Data processing |
Microarray data was analyzed by Expression Console, Transcriptom Analysis Console (Thermo Fisher Scientific) and GeneSpring Software (Agilent).
|
| |
|
| Submission date |
Oct 23, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Marco De Dominici |
| E-mail(s) |
marco.dedominici@jefferson.edu
|
| Phone |
2155034523
|
| Organization name |
Thomas Jefferson University
|
| Department |
Cancer Biology
|
| Lab |
Calabretta
|
| Street address |
10th
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE105826 |
Targeting CDK6 and BCL2 exploits the "MYB Addiction" of Ph+ acute lymphoblastic leukemia |
|
