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Sample GSM2828472 Query DataSets for GSM2828472
Status Public on Jan 17, 2018
Title BV173 doxycycline 2
Sample type RNA
 
Source name cell line BV173
Organism Homo sapiens
Characteristics cell line: BV173
Treatment protocol Cells were plated at 250,000/mL with (DOX) or without (CTRL) doxycycline 1 mg/L for 24 hours
Growth protocol Cells were expanded in Iscove's modified DMEM supplemented with 10% featl bovine serum, 100 U/mL penicicllin/streptomycin and 2 mM L-glutamine
Extracted molecule total RNA
Extraction protocol RNA was extracted from 4-5 millions of cells by using the RNeasy Plus Mini-Kit (Qiagen #74134)
Label biotin
Label protocol biotin labeled cDNA was sythesized using the Affymetrix WT Plus Kit (Thermo Fisher Scientific) for BV173 cells or the Ovation PICO WTA-system V2 and the FL-Ovation 8 cDNA biodin module (NuGen Technologie) for SUP-B15 cells
 
Hybridization protocol cDNA from BV173 cells was hybridized onto Human Clariom S Assay (Affymetrix) and cDNA from SUP-B15 was hybridized onto Human Gene 1.0 ST Array (Affymetrix) folowing the manifacturer instructions.
Scan protocol Arrays were scanned by using the Affymetrix Gene Chip Scanner 3000 using the Command Console Software.
Data processing Microarray data was analyzed by Expression Console, Transcriptom Analysis Console (Thermo Fisher Scientific) and GeneSpring Software (Agilent).
 
Submission date Oct 23, 2017
Last update date Jan 24, 2018
Contact name Marco De Dominici
E-mail(s) marco.dedominici@jefferson.edu
Phone 2155034523
Organization name Thomas Jefferson University
Department Cancer Biology
Lab Calabretta
Street address 10th
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19107
Country USA
 
Platform ID GPL23159
Series (1)
GSE105826 Targeting CDK6 and BCL2 exploits the "MYB Addiction" of Ph+ acute lymphoblastic leukemia

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
AFFX-BkGr-GC03_st 7.23239
AFFX-BkGr-GC04_st 6.76186
AFFX-BkGr-GC05_st 6.74073
AFFX-BkGr-GC06_st 6.59756
AFFX-BkGr-GC07_st 6.274
AFFX-BkGr-GC08_st 3.89279
AFFX-BkGr-GC09_st 3.5402
AFFX-BkGr-GC10_st 3.40427
AFFX-BkGr-GC11_st 3.21236
AFFX-BkGr-GC12_st 3.10494
AFFX-BkGr-GC13_st 2.88005
AFFX-BkGr-GC14_st 2.69963
AFFX-BkGr-GC15_st 2.64927
AFFX-BkGr-GC16_st 2.76372
AFFX-BkGr-GC17_st 2.78786
AFFX-BkGr-GC18_st 3.05393
AFFX-BkGr-GC19_st 5.99537
AFFX-BkGr-GC20_st 6.14077
AFFX-BkGr-GC21_st 6.21434
AFFX-BkGr-GC22_st 6.30528

Total number of rows: 24351

Table truncated, full table size 635 Kbytes.




Supplementary file Size Download File type/resource
GSM2828472_BV173_DOX2.CEL.gz 1.1 Mb (ftp)(http) CEL
GSM2828472_BV173_DOX2.chp.gz 173.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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