|
Status |
Public on Oct 23, 2020 |
Title |
NK biological replicate 2 |
Sample type |
RNA |
|
|
Source name |
NK lymphocyte subset flow sorted by PBMCs
|
Organism |
Homo sapiens |
Characteristics |
cell type: T cell cell subset: NK
|
Treatment protocol |
Cells were not treated in any way
|
Growth protocol |
Cells were flow sorted and frozen immediately upon isolation
|
Extracted molecule |
total RNA |
Extraction protocol |
The RNA was extracted according the RNNeasy Plus Micro Handbook 12/2014 and eluted using 20µl H2O
|
Label |
biotin
|
Label protocol |
The RNA was labeled using 2 ng and the GeneChip Pico Reagent kit and according to AffymetrixÕs protocol
|
|
|
Hybridization protocol |
Hybridization is an integral part of the GeneChip Pico protocol (Cartridge Array Hybridization on the GeneChip¨ Instrument)
|
Scan protocol |
The arrays were scanned on a GeneChip scanner 3000 7G
|
Description |
Gene expression data from human lymphocytes
|
Data processing |
Data was analyzed using the the Affymetrix Expression Console software. Normalization was done by RMA normalization
|
|
|
Submission date |
Oct 24, 2017 |
Last update date |
Oct 23, 2020 |
Contact name |
Mario Galgani |
E-mail(s) |
mario.galgani@unina.it
|
Organization name |
IEOS Istituto per l'endocrinologia e l'oncologia sperimentale
|
Street address |
via S. Pansini, 5
|
City |
Naples |
ZIP/Postal code |
80131 |
Country |
Italy |
|
|
Platform ID |
GPL23159 |
Series (1) |
|