|
| Status |
Public on Oct 23, 2020 |
| Title |
CD8+ biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
CD8+ lymphocyte subset flow sorted by PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: T cell
cell subset: CD8+
|
| Treatment protocol |
Cells were not treated in any way
|
| Growth protocol |
Cells were flow sorted and frozen immediately upon isolation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was extracted according the RNNeasy Plus Micro Handbook 12/2014 and eluted using 20µl H2O
|
| Label |
biotin
|
| Label protocol |
The RNA was labeled using 2 ng and the GeneChip Pico Reagent kit and according to AffymetrixÕs protocol
|
| |
|
| Hybridization protocol |
Hybridization is an integral part of the GeneChip Pico protocol (Cartridge Array Hybridization on the GeneChip¨ Instrument)
|
| Scan protocol |
The arrays were scanned on a GeneChip scanner 3000 7G
|
| Description |
Gene expression data from human lymphocytes
|
| Data processing |
Data was analyzed using the the Affymetrix Expression Console software. Normalization was done by RMA normalization
|
| |
|
| Submission date |
Oct 24, 2017 |
| Last update date |
Oct 23, 2020 |
| Contact name |
Mario Galgani |
| E-mail(s) |
mario.galgani@unina.it
|
| Organization name |
IEOS Istituto per l'endocrinologia e l'oncologia sperimentale
|
| Street address |
via S. Pansini, 5
|
| City |
Naples |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
|
