|
| Status |
Public on Feb 14, 2018 |
| Title |
TIME cells co-cultured with normal fibroblasts, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
TIME cells co-cultured with normal fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TIME
|
| Treatment protocol |
1 x 10^4 TIME human MECs were co-cultured with either 2 x 10^4 primary ovarian CAFs or 2 x 10^4 normal ovarian fibroblasts (NFs) in Boyden chambers for 48 hours
|
| Growth protocol |
Ovarian fibroblasts was cultured with medium M105/199 supplemented with 10% Fetal bovine serum and 1ng/mL Epidermal growth factor. TIME cells were cultured in endothelial cell growth medium-2 (Lonza). All cell types were cultured in a humidified incubator at 37C with 5% carbon dioxide.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using the Purelink RNA mini kit (Invitrogen) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Sample labeling were performed using the Clariom D Pico Assay (Thermo Fisher) with 2ng of total RNA as input according to the manufacturer's instruction.
|
| |
|
| Hybridization protocol |
cRNA samples were hybridized for 16 hr on Clariom D human arrays according to the manufacturer's instruction. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from TIME cells co-cultured with normal fibroblasts
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 03, 2017 |
| Last update date |
Feb 14, 2018 |
| Contact name |
Tsz-Lun Yeung |
| Organization name |
University of Texas MD Anderson Cancer Center
|
| Department |
Gynecologic Oncology and Reproductive Medicine
|
| Street address |
1515 Holcombe Blvd. Unit 1362
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE106519 |
Expression data from human endothelial cells co-cultured with cancer associated fibroblasts |
|
