|
Status |
Public on Jul 01, 2019 |
Title |
OB_WT_6M_R3 |
Sample type |
RNA |
|
|
Source name |
Olfactory bulb, WT, 6m
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: WT tissue: Olfactory bulb gender: Female age: 6 months
|
Treatment protocol |
Not applicable.
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Growth protocol |
Not applicable.
|
Extracted molecule |
total RNA |
Extraction protocol |
For olfactory bulb mRNA extraction, the Maxwell 16 simplyRNA kit (Promega) was used.
|
Label |
biotin
|
Label protocol |
The sense cDNA was prepared from 1 ng of total RNA using the Affymetrix GeneChip® WT Pico Kit (PN902623). The sense strand cDNA was then fragmented and biotinylated according to the manufacturer's instructions.
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|
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Hybridization protocol |
Labeled sense cDNA was hybridized to the GeneChip® Mouse Clariom S Arrays, washed and stained on a GeneChip® Fluidics 450 workstation (Affymetrix) according to the manufacturer protocols.
|
Scan protocol |
GeneChips were scanned with the Affymetrix GeneChip® Scanner 3000 7G.
|
Description |
WT_6M_T2_8672_S11 Affymetrix Mouse Clariom S chip with bioconductor (pd.clariom.s.mouse).
|
Data processing |
Data were normalized with Bioconductor software using RMA algorithm.
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Submission date |
Nov 07, 2017 |
Last update date |
Jul 01, 2019 |
Contact name |
Victor Segura |
E-mail(s) |
vsegura@unav.es
|
Organization name |
FIMA
|
Department |
Unit of Proteomics, Genomics and Bioinformatics
|
Street address |
Avda. Pío XII, 55
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL23038 |
Series (1) |
GSE106643 |
The olfactory molecular snapshot of Tg2576 mice at early stages of amyloid pathology |
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