|
| Status |
Public on Jun 01, 2008 |
| Title |
A2B5/OTMP+ chipB biological replicate-2 |
| Sample type |
RNA |
| |
|
| Source name |
A2B5/OTMP+ oligodendrocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
Fluorescent activated cell sorted A2B5/OTMP+ oligodendrocytes isolated from post-natal day 7 rat whole brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
biotin
|
| Label protocol |
Affymetrix small sample labeling protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocols were followed using the hybridization oven 640, and washing with the fluidics station 450
|
| Scan protocol |
The microarrays were scanned with a genchip scanner 3000 7G
|
| Description |
Flow Cytometry was used to isolate A2B5/OTMP+ oligodendrocytes from postnatal day 7 rat brain. 4 bioloical replicates were obtained. This data can be compared to the previously submitted oligodendrocyte expression data (GSE5940).
|
| Data processing |
Cel files were imported into Genespring for preprocessing using RMA, and normalization using a global per-chip and per-gene normalization strategy
|
| |
|
| Submission date |
Apr 27, 2008 |
| Last update date |
Apr 28, 2008 |
| Contact name |
Joe A Nielsen |
| E-mail(s) |
nielsenj@ninds.nih.gov
|
| Phone |
301-402-7350
|
| Organization name |
NIH/NINDS
|
| Lab |
Section of Develpmental Genetics
|
| Street address |
49 Convent Drive BD49,5A75
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-4479 |
| Country |
USA |
| |
|
| Platform ID |
GPL342 |
| Series (1) |
| GSE11277 |
A2B5/OTMP+ rat perineuronal oligodendrocytes |
|
