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GEO help: Mouse over screen elements for information. |
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Status |
Public on Apr 29, 2008 |
Title |
WAT_WT_HFD |
Sample type |
RNA |
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Source name |
white adipose tissue
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Organism |
Mus musculus |
Characteristics |
129S1/SvImJ, Male
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Extracted molecule |
total RNA |
Extraction protocol |
Epididymal adipose tissue was excised, homogenized in TRIzol reagent. RNA isolated was further purified using Qiagen RNEasy columns.
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Label |
biotin
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Label protocol |
The RNA (5µg) was labled using the Affymetrix One-cycle Target Labeling Assay kit, according to the manufacteror's instructions.
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Hybridization protocol |
Arrays were hybridized according to the standard protocols of Affymetrix (16 hours at 42 degrees centigrade)
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Scan protocol |
Arrays were scanned on an Affymetrix GeneChip scanner 3000 7G, according to the standard protocols of Affymetrix
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Description |
A794WTHFD
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Data processing |
Expression estimates were calculated using the multi-chip modified gamma Model for Oligonucleotide Signal (multi-mgMOS) [PMID: 16020470]
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Submission date |
Apr 29, 2008 |
Last update date |
Apr 29, 2008 |
Contact name |
Guido Hooiveld |
E-mail(s) |
guido.hooiveld@wur.nl
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Organization name |
Wageningen University
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Department |
Div. Human Nutrition & Health
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Lab |
Nutrition, Metabolism & Genomics Group
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Street address |
HELIX, Stippeneng 4
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City |
Wageningen |
ZIP/Postal code |
NL-6708WE |
Country |
Netherlands |
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Platform ID |
GPL5008 |
Series (1) |
GSE11295 |
PPARγ activation promotes infiltration of alternatively activated macrophages into adipose tissue. |
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Data table header descriptions |
ID_REF |
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VALUE |
mmgMOS signal (=log2 expression value) |
Data table |
ID_REF |
VALUE |
100012_at |
-1.921000907 |
100017_at |
5.777264614 |
100019_at |
4.345780244 |
100061_at |
1.072965448 |
100066_at |
4.595910805 |
100072_at |
3.601486773 |
100087_at |
7.286431319 |
100088_at |
4.422473196 |
100090_at |
4.823880995 |
100102_at |
-0.665746145 |
100121_at |
9.208417945 |
100129_at |
4.183900933 |
100163_at |
6.984091759 |
100169_at |
8.272016843 |
100177_at |
6.435317184 |
100198_at |
9.430330125 |
100201_at |
7.055607521 |
100206_at |
5.69715874 |
100210_at |
5.402448714 |
100213_at |
6.275459743 |
Total number of rows: 16642
Table truncated, full table size 347 Kbytes.
Supplementary file |
Size |
Download |
File type/resource |
GSM285058.CEL.gz |
3.4 Mb |
(ftp)(http) |
CEL |
Processed data included within Sample table |
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