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Sample GSM285058 Query DataSets for GSM285058
Status Public on Apr 29, 2008
Title WAT_WT_HFD
Sample type RNA
 
Source name white adipose tissue
Organism Mus musculus
Characteristics 129S1/SvImJ, Male
Extracted molecule total RNA
Extraction protocol Epididymal adipose tissue was excised, homogenized in TRIzol reagent. RNA isolated was further purified using Qiagen RNEasy columns.
Label biotin
Label protocol The RNA (5µg) was labled using the Affymetrix One-cycle Target Labeling Assay kit, according to the manufacteror's instructions.
 
Hybridization protocol Arrays were hybridized according to the standard protocols of Affymetrix (16 hours at 42 degrees centigrade)
Scan protocol Arrays were scanned on an Affymetrix GeneChip scanner 3000 7G, according to the standard protocols of Affymetrix
Description A794WTHFD
Data processing Expression estimates were calculated using the multi-chip modified gamma Model for Oligonucleotide Signal (multi-mgMOS) [PMID: 16020470]
 
Submission date Apr 29, 2008
Last update date Apr 29, 2008
Contact name Guido Hooiveld
E-mail(s) guido.hooiveld@wur.nl
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
 
Platform ID GPL5008
Series (1)
GSE11295 PPARγ activation promotes infiltration of alternatively activated macrophages into adipose tissue.

Data table header descriptions
ID_REF
VALUE mmgMOS signal (=log2 expression value)

Data table
ID_REF VALUE
100012_at -1.921000907
100017_at 5.777264614
100019_at 4.345780244
100061_at 1.072965448
100066_at 4.595910805
100072_at 3.601486773
100087_at 7.286431319
100088_at 4.422473196
100090_at 4.823880995
100102_at -0.665746145
100121_at 9.208417945
100129_at 4.183900933
100163_at 6.984091759
100169_at 8.272016843
100177_at 6.435317184
100198_at 9.430330125
100201_at 7.055607521
100206_at 5.69715874
100210_at 5.402448714
100213_at 6.275459743

Total number of rows: 16642

Table truncated, full table size 347 Kbytes.




Supplementary file Size Download File type/resource
GSM285058.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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