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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 29, 2008 |
| Title |
WAT_WT_HFD+Rosi |
| Sample type |
RNA |
| |
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| Source name |
white adipose tissue
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| Organism |
Mus musculus |
| Characteristics |
129S1/SvImJ, Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Epididymal adipose tissue was excised, homogenized in TRIzol reagent. RNA isolated was further purified using Qiagen RNEasy columns.
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| Label |
biotin
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| Label protocol |
The RNA (5µg) was labled using the Affymetrix One-cycle Target Labeling Assay kit, according to the manufacteror's instructions.
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| Hybridization protocol |
Arrays were hybridized according to the standard protocols of Affymetrix (16 hours at 42 degrees centigrade)
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| Scan protocol |
Arrays were scanned on an Affymetrix GeneChip scanner 3000 7G, according to the standard protocols of Affymetrix
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| Description |
A798WTHFDrosi
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| Data processing |
Expression estimates were calculated using the multi-chip modified gamma Model for Oligonucleotide Signal (multi-mgMOS) [PMID: 16020470]
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| Submission date |
Apr 29, 2008 |
| Last update date |
Apr 29, 2008 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platform ID |
GPL5008 |
| Series (1) |
| GSE11295 |
PPARγ activation promotes infiltration of alternatively activated macrophages into adipose tissue. |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
mmgMOS signal (=log2 expression value) |
| Data table |
| ID_REF |
VALUE |
| 100012_at |
-5.713236775 |
| 100017_at |
6.117879923 |
| 100019_at |
5.235493513 |
| 100061_at |
0.175413023 |
| 100066_at |
2.296917951 |
| 100072_at |
2.758830206 |
| 100087_at |
7.708852871 |
| 100088_at |
5.289491928 |
| 100090_at |
4.710930737 |
| 100102_at |
-2.816531448 |
| 100121_at |
9.560298983 |
| 100129_at |
5.356412587 |
| 100163_at |
7.58897429 |
| 100169_at |
7.76546447 |
| 100177_at |
6.19904584 |
| 100198_at |
8.824441594 |
| 100201_at |
7.88212884 |
| 100206_at |
6.157160178 |
| 100210_at |
5.606043679 |
| 100213_at |
5.962181411 |
Total number of rows: 16642
Table truncated, full table size 347 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM285059.CEL.gz |
3.5 Mb |
(ftp)(http) |
CEL |
| Processed data included within Sample table |
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