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Sample GSM285612 Query DataSets for GSM285612
Status Public on Jun 05, 2008
Title hESC_KSR_control_1
Sample type RNA
 
Source name NIH WA-09 (H9 hESCs)
Organism Homo sapiens
Characteristics status after 24hrs treatment
KSR (serum-replacement medium 15%)
Differentiation Assay
control
Extracted molecule total RNA
Extraction protocol total RNA was extracted from cell pellet using Trizol (invitrogen)
Label biotin
Label protocol 200ng ot total RNA were labeled using the Illumina Total Prep RNA Amplification Kit (Ambion, cat#AMIL1791) according the manufacturer's instructions
 
Hybridization protocol 750ng of labeled cRNA were hybridized on the Illumina Human Ref-8 V2.0 arrays according to Illumina's instructions.
Scan protocol according to manufacturer
Description Illumina human ref-8V2.0
Data processing The raw gene level Signal's where normalized using the standard quantile normalization from the bioconductor limma package. Then the data was transformed using the generalized logarithm function glog(x)=(asinh(x)-log(2))/log(2). Note: the (1/log(2)) term makes this glog asymptotically equivalent to log base 2.
 
Submission date Apr 30, 2008
Last update date Jun 05, 2008
Contact name Agnes Viale
E-mail(s) vialea@mskcc.org
Phone 212 639 3031
Fax 212 717 3053
Organization name Memorial Sloan Kettering Cancer Center
Department Molecular Biology
Lab Genomics Core Laboratory
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL6104
Series (1)
GSE11302 Gene expression analysis upon exposure of hESCs to novel set of self-renewal and differentiation compounds

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
ILMN_1698220 8.71856114938045
ILMN_1810835 5.84379375500302
ILMN_1782944 6.38918294255736
ILMN_1692858 6.10283111003776
ILMN_1668162 6.61896086253569
ILMN_1682799 8.94216543712791
ILMN_1696317 5.94386368035548
ILMN_1669181 5.7732719688585
ILMN_1665311 6.2502016190607
ILMN_1734631 6.30899660861552
ILMN_1711453 6.19335603820795
ILMN_1784717 14.5794427538843
ILMN_1702928 6.51708025376901
ILMN_1667120 6.08456633009165
ILMN_1761911 8.36594508479217
ILMN_1706784 6.42474086307787
ILMN_1751493 6.01023816708053
ILMN_1815346 9.16503956557957
ILMN_1774687 6.51971225549278
ILMN_1764927 7.5295442776761

Total number of rows: 22185

Table truncated, full table size 646 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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