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Sample GSM285628 Query DataSets for GSM285628
Status Public on Jun 05, 2008
Title hESC_HES_GTFX_2
Sample type RNA
 
Source name NIH WA-09 (H9 hESCs)
Organism Homo sapiens
Characteristics status after 24hrs treatment
HES (hESC medium)
Self-renewal Assay
GTFX
Extracted molecule total RNA
Extraction protocol total RNA was extracted from cell pellet using Trizol (invitrogen)
Label biotin
Label protocol 200ng ot total RNA were labeled using the Illumina Total Prep RNA Amplification Kit (Ambion, cat#AMIL1791) according the manufacturer's instructions
 
Hybridization protocol 750ng of labeled cRNA were hybridized on the Illumina Human Ref-8 V2.0 arrays according to Illumina's instructions.
Scan protocol according to manufacturer
Description Illumina human ref-8V2.0
Data processing The raw gene level Signal's where normalized using the standard quantile normalization from the bioconductor limma package. Then the data was transformed using the generalized logarithm function glog(x)=(asinh(x)-log(2))/log(2). Note: the (1/log(2)) term makes this glog asymptotically equivalent to log base 2.
 
Submission date Apr 30, 2008
Last update date Jun 05, 2008
Contact name Agnes Viale
E-mail(s) vialea@mskcc.org
Phone 212 639 3031
Fax 212 717 3053
Organization name Memorial Sloan Kettering Cancer Center
Department Molecular Biology
Lab Genomics Core Laboratory
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL6104
Series (1)
GSE11302 Gene expression analysis upon exposure of hESCs to novel set of self-renewal and differentiation compounds

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
ILMN_1698220 8.70438443969509
ILMN_1810835 5.95337970266504
ILMN_1782944 6.30090254827368
ILMN_1692858 6.00439418487412
ILMN_1668162 6.29907584416491
ILMN_1682799 8.68985088044385
ILMN_1696317 6.14800795714178
ILMN_1669181 5.65866983461531
ILMN_1665311 6.14216454266054
ILMN_1734631 6.15071179395107
ILMN_1711453 5.91004160472869
ILMN_1784717 14.5406418520091
ILMN_1702928 6.2814472607304
ILMN_1667120 5.71104199727044
ILMN_1761911 8.24320800041372
ILMN_1706784 6.36647277129024
ILMN_1751493 5.913136761489
ILMN_1815346 8.985882888663
ILMN_1774687 6.3510352647332
ILMN_1764927 6.98111761230937

Total number of rows: 22185

Table truncated, full table size 646 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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