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Sample GSM285632 Query DataSets for GSM285632
Status Public on Jun 05, 2008
Title hESC_HES_control_3
Sample type RNA
 
Source name NIH WA-09 (H9 hESCs)
Organism Homo sapiens
Characteristics status after 24hrs treatment
HES (hESC medium)
Self-renewal Assay
control
Extracted molecule total RNA
Extraction protocol total RNA was extracted from cell pellet using Trizol (invitrogen)
Label biotin
Label protocol 200ng ot total RNA were labeled using the Illumina Total Prep RNA Amplification Kit (Ambion, cat#AMIL1791) according the manufacturer's instructions
 
Hybridization protocol 750ng of labeled cRNA were hybridized on the Illumina Human Ref-8 V2.0 arrays according to Illumina's instructions.
Scan protocol according to manufacturer
Description Illumina human ref-8V2.0
Data processing The raw gene level Signal's where normalized using the standard quantile normalization from the bioconductor limma package. Then the data was transformed using the generalized logarithm function glog(x)=(asinh(x)-log(2))/log(2). Note: the (1/log(2)) term makes this glog asymptotically equivalent to log base 2.
 
Submission date Apr 30, 2008
Last update date Jun 05, 2008
Contact name Agnes Viale
E-mail(s) vialea@mskcc.org
Phone 212 639 3031
Fax 212 717 3053
Organization name Memorial Sloan Kettering Cancer Center
Department Molecular Biology
Lab Genomics Core Laboratory
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL6104
Series (1)
GSE11302 Gene expression analysis upon exposure of hESCs to novel set of self-renewal and differentiation compounds

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
ILMN_1698220 8.90584046919314
ILMN_1810835 6.03702396268131
ILMN_1782944 6.45507678434181
ILMN_1692858 6.06453820156568
ILMN_1668162 6.42595618720534
ILMN_1682799 8.85198650446492
ILMN_1696317 6.07030432732022
ILMN_1669181 5.51099898804873
ILMN_1665311 5.97129872845823
ILMN_1734631 6.50106090565543
ILMN_1711453 5.95655835950694
ILMN_1784717 14.4146255993630
ILMN_1702928 6.42533185984356
ILMN_1667120 5.82706607691352
ILMN_1761911 8.00276920610936
ILMN_1706784 6.2968509591957
ILMN_1751493 6.03715102863476
ILMN_1815346 9.07448810955465
ILMN_1774687 6.335262973044
ILMN_1764927 7.8043373157689

Total number of rows: 22185

Table truncated, full table size 646 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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