|
| Status |
Public on May 01, 2008 |
| Title |
DS-2-1 |
| Sample type |
RNA |
| |
|
| Source name |
cultured stromal cell line 2
|
| Organism |
Canis lupus familiaris |
| Characteristics |
stromal cell line
|
| Growth protocol |
Cloned cell lines were cultured in 10 cm plates for 2-3 days to 80% confluency in RPMI1640 medium supplemented with 10% FBS/2mM glutamine at 37ºC in 5% CO2 . Cells were trypsinized, centrifuged and lysed or lysed directly in the plate with Qiagen RLT buffer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Qiagen RNeasy kits according to manufacturer's protocols.
|
| Label |
biotin
|
| Label protocol |
cRNA was prepared from 8-10 ug total RNA according to Affymetrix protocols using the SuperScriptChoice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol and reagents
|
| Scan protocol |
standard Affymetrix protocol and instruments
|
| Description |
dog stromal cell line 2, cultured, biological replicate 1
|
| Data processing |
Affymetrix CEL files were imported into GeneSpring 7.3.1 and samples were preprocessed with GC-RMA to obtain the normalized natural hybridization values. The Per gene normalization feature of Genespring, in which each measurement is divided by the median of its measurements in all simples, was also applied.
|
| |
|
| Submission date |
Apr 30, 2008 |
| Last update date |
Apr 30, 2008 |
| Contact name |
Lynn Graf |
| E-mail(s) |
lgraf@fhcrc.org
|
| Phone |
206-667-4545
|
| Fax |
206-667-5978
|
| URL |
http://none
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Clinical Research Division
|
| Lab |
Torok-Storb
|
| Street address |
1100 Fairview Ave. N. D1-100
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE11310 |
Characterization of canine mesenchymal stromal cell lines (MSC) |
|
