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Sample GSM285750 Query DataSets for GSM285750
Status Public on May 01, 2008
Title DS-3-1
Sample type RNA
 
Source name cultured stromal cell line 3
Organism Canis lupus familiaris
Characteristics stromal cell line
Growth protocol Cloned cell lines were cultured in 10 cm plates for 2-3 days to 80% confluency in RPMI1640 medium supplemented with 10% FBS/2mM glutamine at 37ÂșC in 5% CO2 . Cells were trypsinized, centrifuged and lysed or lysed directly in the plate with Qiagen RLT buffer.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Qiagen RNeasy kits according to manufacturer's protocols.
Label biotin
Label protocol cRNA was prepared from 8-10 ug total RNA according to Affymetrix protocols using the SuperScriptChoice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
 
Hybridization protocol standard Affymetrix protocol and reagents
Scan protocol standard Affymetrix protocol and instruments
Description dog stromal cell line 3, cultured, biological replicate 1
Data processing Affymetrix CEL files were imported into GeneSpring 7.3.1 and samples were preprocessed with GC-RMA to obtain the normalized natural hybridization values. The Per gene normalization feature of Genespring, in which each measurement is divided by the median of its measurements in all simples, was also applied.
 
Submission date Apr 30, 2008
Last update date Apr 30, 2008
Contact name Lynn Graf
E-mail(s) lgraf@fhcrc.org
Phone 206-667-4545
Fax 206-667-5978
URL http://none
Organization name Fred Hutchinson Cancer Research Center
Department Clinical Research Division
Lab Torok-Storb
Street address 1100 Fairview Ave. N. D1-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL3738
Series (1)
GSE11310 Characterization of canine mesenchymal stromal cell lines (MSC)

Data table header descriptions
ID_REF
VALUE Values are GC-RMA normalized.

Data table
ID_REF VALUE
AFFX-BioB-3_at 357
AFFX-BioB-5_at 507
AFFX-BioB-M_at 1273
AFFX-BioC-3_at 1597
AFFX-BioC-5_at 1107
AFFX-BioDn-3_at 8059
AFFX-BioDn-5_at 5288
AFFX-Cfa-actin-3_s_at 13302
AFFX-Cfa-actin-5_at 897
AFFX-Cfa-actin-5_s_at 6279
AFFX-Cfa-actin-M_x_at 37248
AFFX-Cfa-ef1a-3_x_at 36645
AFFX-Cfa-ef1a-5_at 11
AFFX-Cfa-ef1a-M_x_at 25192
AFFX-Cfa-gapdh-3_x_at 43119
AFFX-Cfa-gapdh-5_x_at 6643
AFFX-Cfa-gapdh-M_x_at 26867
AFFX-Cfa-r2-Bs-dap-3_at 6
AFFX-Cfa-r2-Bs-dap-5_at 6
AFFX-Cfa-r2-Bs-dap-M_at 6

Total number of rows: 43035

Table truncated, full table size 1001 Kbytes.




Supplementary file Size Download File type/resource
GSM285750.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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