|
| Status |
Public on Jun 04, 2019 |
| Title |
Negative control (HpsPr-WC)-24h, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
PC3 prostate cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC3
cell type: prostate cancer
|
| Treatment protocol |
Incubation with 100 nM of either HpsPr-WC or HpsPr-WT for 24 hours.
|
| Growth protocol |
PC3 human prostate cancer cells (30,000) were seeded in 35 mm plates, grown in Ham's F12 medium supplemented with 7% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction with TRI reagent. extraction performed according to user's protocol.
|
| Label |
Biotin
|
| Label protocol |
Fragmented and labeled ss-cDNA was prepared according to Affymetrix WT PLUS Reagent Kit user guide
|
| |
|
| Hybridization protocol |
Following fragmentation and terminal labeling, 5.2ug of ss-cDNA were hybridized for 16hr at 45ºC on GeneChip Human Clariom S array. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneCHip Scanner 3000 System.
|
| Description |
Gene expression from 24h treatment with negative control (HpsPr-WC)
|
| Data processing |
Data was processed using Genespring v14.5 software, computing the RMA (Robust Multichip Average) expression measure in log2 base scale, using a baseline transformation to the median of control samples
Affymetrix.TranscriptomeChip.Clariom_S_Human_na36_hg38_2016-06-21
|
| |
|
| Submission date |
Nov 17, 2017 |
| Last update date |
Jun 04, 2019 |
| Contact name |
Carlos J Ciudad |
| E-mail(s) |
cciudad@ub.edu
|
| Phone |
+34-93-403-4455
|
| Organization name |
University of Barcelona
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
School of Pharmacy
|
| Street address |
Av. Juan XXIII-27
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE107106 |
Analysis of gene expression of PC3 human prostate cancer cells incubated with Polypurine reverse Hoogsteen hairpins (PPRH) against survivin |
|
