|
| Status |
Public on Mar 13, 2018 |
| Title |
Liver Tumor from Axin1 deleted mouse N°124 |
| Sample type |
RNA |
| |
|
| Source name |
Liver tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
age: 16 months
gender: male
|
| Treatment protocol |
Mutant mice were obtained by breeding Axin1 fl/fl mice with mice expressing Cre recombinase in embryonic hepatocytes (Alfp-Cre). Axin1-/- mice develop liver tumors after 9 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
50 ng of total RNA is reverse transcribed following the Ovation PicoSL WTA System V2 (Nugen). Briefly, the resulting double strand cDNA is used for amplification based on SPIA technology. After purification according to Nugen protocol, 2.5 ug of single strand DNA is used for fragmentation and biotin labelling using Encore Biotin Module (Nugen).
|
| |
|
| Hybridization protocol |
biotynilated single stranded sense cDNA is then hybridized to GeneChip® Mouse Gene2.0 (Affymetrix) at 45°C for 17 hours
|
| Scan protocol |
After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix) and scanned using the GCS3000 7G
|
| Description |
Gene expression data
|
| Data processing |
Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation. We ve used the custom CDF file from BrainArray : mogene20st_Mm_ENTREZG V18.
|
| |
|
| Submission date |
Nov 27, 2017 |
| Last update date |
Mar 13, 2018 |
| Contact name |
Franck Letourneur |
| E-mail(s) |
franck.letourneur@inserm.fr
|
| Organization name |
INSERM
|
| Department |
U1016
|
| Lab |
genomic
|
| Street address |
22 rue mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL18802 |
| Series (1) |
| GSE107374 |
Expression data from mouse hepatocellular carcinomas developed in Axin1 hepatocyte deleted mice |
|
