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Sample GSM2865622 Query DataSets for GSM2865622
Status Public on Mar 13, 2018
Title Liver Tumor from Axin1 deleted mouse N°81
Sample type RNA
Source name Liver tumor
Organism Mus musculus
Characteristics tissue: Liver
age: 16 months
gender: male
Treatment protocol Mutant mice were obtained by breeding Axin1 fl/fl mice with mice expressing Cre recombinase in embryonic hepatocytes (Alfp-Cre). Axin1-/- mice develop liver tumors after 9 months
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol 50 ng of total RNA is reverse transcribed following the Ovation PicoSL WTA System V2 (Nugen). Briefly, the resulting double strand cDNA is used for amplification based on SPIA technology. After purification according to Nugen protocol, 2.5 ug of single strand DNA is used for fragmentation and biotin labelling using Encore Biotin Module (Nugen).
Hybridization protocol biotynilated single stranded sense cDNA is then hybridized to GeneChip® Mouse Gene2.0 (Affymetrix) at 45°C for 17 hours
Scan protocol After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix) and scanned using the GCS3000 7G
Description Gene expression data
Data processing Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation. We ve used the custom CDF file from BrainArray : mogene20st_Mm_ENTREZG V18.
Submission date Nov 27, 2017
Last update date Mar 13, 2018
Contact name Franck Letourneur
Organization name INSERM
Department U1016
Lab genomic
Street address 22 rue mechain
City Paris
ZIP/Postal code 75014
Country France
Platform ID GPL18802
Series (1)
GSE107374 Expression data from mouse hepatocellular carcinomas developed in Axin1 hepatocyte deleted mice

Data table header descriptions
VALUE Log2-RMA signal

Data table
100008567_at 5.004579862
100009600_at 3.714345345
100009609_at 1.97207437
100009614_at 2.952795362
100009664_at 3.026140535
100012_at 3.669299202
100017_at 5.882780891
100019_at 5.531621074
100033459_at 3.455409933
100034251_at 8.324246951
100034729_at 3.814061056
100034739_at 3.287581954
100034748_at 3.561498277
100036518_at 2.219297301
100036520_at 3.430222582
100036521_at 3.958437522
100036523_at 5.595561855
100036537_at 3.477591159
100036768_at 4.159070382
100037258_at 9.85685851

Total number of rows: 24479

Table truncated, full table size 515 Kbytes.

Supplementary file Size Download File type/resource
GSM2865622_9_A81_T_MoGene-2_0-st_.CEL.gz 8.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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