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Sample GSM2867912 Query DataSets for GSM2867912
Status Public on Nov 30, 2018
Title Control skin biopsy 3
Sample type RNA
 
Source name Control skin biopsy
Organism Homo sapiens
Characteristics gender: Female
age: 57
disease state: control
tissue: Skin biopsy (mainly epidermis)
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TriReagent (Ambion) according to the protocol.
Label Biotin
Label protocol 250 nanograms of total RNA from each sample were used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip® WT PLUS Reagent Kit User Manual ( P/N 703174 Rev 1 Affymetrix Inc., Santa Clara, CA)
 
Hybridization protocol GeneChip® Clariom D Human Arrays were hybridized for 16 hours in a 45°C incubator, followed by washing and staining
Scan protocol The GeneChip® Array-plates were scanned with GeneTitan® Multi-Channel (MC) Instrument
Data processing The raw data was normalized in Expression Console provided by Affymetrix, using the robust multi-array average (RMA) method.
 
Submission date Nov 29, 2017
Last update date Nov 30, 2018
Contact name Hans Törmä
E-mail(s) hans.torma@medsci.uu.se
Organization name Uppsala University
Department Dept of Medical Sciences
Lab Rudbeck Laboratory/Dermatology
Street address Dag Hammarskjölds väg 20
City Uppsala
ZIP/Postal code 75185
Country Sweden
 
Platform ID GPL23126
Series (1)
GSE107462 Patients with Congenital Ichthyosis and TGM1 Mutations Overexpress Other ARCI Genes in the Skin: Part of a Barrier Repair Response?

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
AFFX-BkGr-GC03_st 9.70872
AFFX-BkGr-GC04_st 8.22859
AFFX-BkGr-GC05_st 6.92302
AFFX-BkGr-GC06_st 6.13775
AFFX-BkGr-GC07_st 5.36903
AFFX-BkGr-GC08_st 4.78807
AFFX-BkGr-GC09_st 4.10193
AFFX-BkGr-GC10_st 3.61077
AFFX-BkGr-GC11_st 3.21187
AFFX-BkGr-GC12_st 3.00209
AFFX-BkGr-GC13_st 2.68977
AFFX-BkGr-GC14_st 2.54555
AFFX-BkGr-GC15_st 2.47054
AFFX-BkGr-GC16_st 2.62883
AFFX-BkGr-GC17_st 2.6184
AFFX-BkGr-GC18_st 2.82521
AFFX-BkGr-GC19_st 3.31639
AFFX-BkGr-GC20_st 3.42057
AFFX-BkGr-GC21_st 3.58089
AFFX-BkGr-GC22_st 4.31418

Total number of rows: 138745

Table truncated, full table size 3533 Kbytes.




Supplementary file Size Download File type/resource
GSM2867912_PA292_Control_NHS_3_20170217_Clariom_D_Human_.CEL.gz 25.0 Mb (ftp)(http) CEL
GSM2867912_PA292_Control_NHS_3_20170217_Clariom_D_Human_.sst-rma-gene-full.chp.gz 891.5 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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