|
| Status |
Public on Nov 30, 2018 |
| Title |
ARCI skin biopsy P2 |
| Sample type |
RNA |
| |
|
| Source name |
ARCI skin biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 38
disease state: ARCI
tissue: Skin biopsy (mainly epidermis)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TriReagent (Ambion) according to the protocol.
|
| Label |
Biotin
|
| Label protocol |
250 nanograms of total RNA from each sample were used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip® WT PLUS Reagent Kit User Manual ( P/N 703174 Rev 1 Affymetrix Inc., Santa Clara, CA)
|
| |
|
| Hybridization protocol |
GeneChip® Clariom D Human Arrays were hybridized for 16 hours in a 45°C incubator, followed by washing and staining
|
| Scan protocol |
The GeneChip® Array-plates were scanned with GeneTitan® Multi-Channel (MC) Instrument
|
| Data processing |
The raw data was normalized in Expression Console provided by Affymetrix, using the robust multi-array average (RMA) method.
|
| |
|
| Submission date |
Nov 29, 2017 |
| Last update date |
Nov 30, 2018 |
| Contact name |
Hans Törmä |
| E-mail(s) |
hans.torma@medsci.uu.se
|
| Organization name |
Uppsala University
|
| Department |
Dept of Medical Sciences
|
| Lab |
Rudbeck Laboratory/Dermatology
|
| Street address |
Dag Hammarskjölds väg 20
|
| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE107462 |
Patients with Congenital Ichthyosis and TGM1 Mutations Overexpress Other ARCI Genes in the Skin: Part of a Barrier Repair Response? |
|
