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Sample GSM287164 Query DataSets for GSM287164
Status Public on Jun 18, 2008
Title umbilical_cord_blood_CD38-
Sample type SAGE
Anchor NlaIII
Tag Count 191931
Tag Length 17
 
Source name Human umbilical cord blood stem/progenitor cells
Organism Homo sapiens
Characteristics Tissue: umbilical cord blood
Gender: Male/Female
Estimated 6% colony forming cells and 14% lonterm culture-initiating cells
Cell purification method: Immuno-magnetic and Fluorescent Activated Cell Sorting (FACS)
Defining markers: lin-CD34+CD38-CD45RA-CD36-CD71-CD7- (lin = CD2, CD3, CD14, CD16, CD19, CD20, CD24, CD56, CD66b and GlyA)
Biomaterial provider Dr. Connie Eaves (ceaves@bccrc.ca), BC Cancer Research Centre
Extracted molecule total RNA
Extraction protocol PicoPureTM (Arcturus, Mountain View, CA)
 
Description Assays to confirm viability: Colony Forming Cell Assay (CFCs) / Longterm Culture-Initiating Cells (LTC-ICs)
PCR amplification before SAGE
 
Submission date May 06, 2008
Last update date Nov 19, 2008
Contact name BCGSC BC Cancer Agency
E-mail(s) support@mouseatlas.org
Organization name Canada's Michael Smith Genome Sciences Centre
Department Gene Expression
Lab Marco Marra
Street address 675 West 10th Avenue
City Vancouver
State/province BC
ZIP/Postal code V5Z 1L3
Country Canada
 
Platform ID GPL1485
Series (1)
GSE11795 Molecular Profiling of Primitive Hematopoietic Cells Generated In Vitro from Human Embryonic Stem Cells and In Vivo during Embryogenesis

Data table header descriptions
TAG
COUNT
TPM tags per million

Data table
TAG COUNT TPM
AAAAAAAAAAAAAAAAA 34 177.15
AAAAAAAAAAAAAAAAG 4 20.84
AAAAAAAAAAAAAAAAT 2 10.42
AAAAAAAAAAAAAAGAG 1 5.21
AAAAAAAAAAAAACAAA 1 5.21
AAAAAAAAAAAAAGTAG 1 5.21
AAAAAAAAAAAAGAAAA 2 10.42
AAAAAAAAAAAAGCCAA 1 5.21
AAAAAAAAAAAGAAAAA 1 5.21
AAAAAAAAAAAGAAAAG 2 10.42
AAAAAAAAAATACAAAA 1 5.21
AAAAAAAAAGACAATTA 1 5.21
AAAAAAAAGAAAAAAAA 2 10.42
AAAAAAAAGAAGTTTTG 1 5.21
AAAAAAAATTTCCAATG 1 5.21
AAAAAAACTATGCTGCT 1 5.21
AAAAAAAGAACTCCACT 1 5.21
AAAAAAAGAAGAAAAGG 1 5.21
AAAAAAAGCAAAAAAAA 2 10.42
AAAAAAAGCTCATCATC 1 5.21

Total number of rows: 53283

Table truncated, full table size 1319 Kbytes.




Supplementary data files not provided

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