|
| Status |
Public on Oct 01, 2008 |
| Title |
Arbidopsis_30_minutes_low_temp |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
65416 |
| Tag Length |
10 |
| |
|
| Source name |
Arabidopsis thaliana: 30 minutes, low temp
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Arabidopsis thaliana (Col-4)
Aerial tissues collected from 14-day old plants. Grown under axenic conditions at 22 degrees Celsius and 125 uE light with a 16 hour photo-period on 0.5 x MS media.
Exposed to 4 degrees Celsius for 30 minutes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 10 g of Arabidopsis plant tissue using the Total RNA extraction kit and mRNA was purified from total RNA using an mRNA purification kit according to the manufacturer’s protocols (Amersham Biosciences, Baie d’Urfe, Canada).
|
| |
|
| Description |
Characterization of the gene expression changes occurring throughout the cold acclimation adaptation.
30 minutes of low temperature library.
|
| |
|
| Submission date |
May 07, 2008 |
| Last update date |
Nov 19, 2008 |
| Contact name |
Stephen James Robinson |
| E-mail(s) |
robinsons@agr.gc.ca
|
| Phone |
306-956-2843
|
| Fax |
306-9567247
|
| Organization name |
Agriculture and agri-Food Canada
|
| Department |
Molecular Genetics
|
| Street address |
107 Science Place
|
| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7H 0X2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL25 |
| Series (1) |
| GSE11461 |
Differential SAGE analysis in Arabidopsis uncovers increased transcriptome complexity in response to low temperature |
|
