NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM287587 Query DataSets for GSM287587
Status Public on May 10, 2008
Title Diff-Basal
Sample type SAGE
Anchor NlaIII
Tag Count 163472
Tag Length 17
 
Source name Differentiated basal Cells of mammary tissue
Organism Homo sapiens
Characteristics Tissue: reduction mammoplasty sample
Gender: Female
Age: 26 days
Biomaterial provider Dr. Connie Eaves (ceaves@bccrc.ca), BC Cancer Research Centre
Treatment protocol Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspensions and co-cultured with irradiated mouse fibroblasts for 3 days.
Growth protocol Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
 
Description Highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal human mammary tissue were isolated and compared their transcriptomes obtained using PCR-LONG-SAGE protocol (Zhao, et al, Stem Cells, 2007)
 
Submission date May 08, 2008
Last update date Nov 19, 2008
Contact name BCGSC BC Cancer Agency
E-mail(s) support@mouseatlas.org
Organization name Canada's Michael Smith Genome Sciences Centre
Department Gene Expression
Lab Marco Marra
Street address 675 West 10th Avenue
City Vancouver
State/province BC
ZIP/Postal code V5Z 1L3
Country Canada
 
Platform ID GPL1485
Series (2)
GSE11387 Transcript Analysis of Human Mammary Cell Subsets using SAGE
GSE11395 Transcript Analysis of Human Mammary Cell Subsets

Data table header descriptions
TAG
COUNT
TPM tags per million

Data table
TAG COUNT TPM
AAAAAAAAAAAAAAAAA 80 489.38
AAAAAAAAAAAAAAAAC 7 42.82
AAAAAAAAAAAAAAAAG 3 18.35
AAAAAAAAAAAAAAAAT 3 18.35
AAAAAAAAAAAAAAACG 1 6.12
AAAAAAAAAAAAAAAGA 1 6.12
AAAAAAAAAAAAAAATA 1 6.12
AAAAAAAAAAAAAAATT 1 6.12
AAAAAAAAAAAAAAGTT 1 6.12
AAAAAAAAAAAAAGAGA 1 6.12
AAAAAAAAAAACAAAAA 1 6.12
AAAAAAAAAAAGTAGCA 1 6.12
AAAAAAAAAACAAAAAC 1 6.12
AAAAAAAAAAGAAAAAA 1 6.12
AAAAAAAAAATAGAAAA 1 6.12
AAAAAAAAAATCTTGCA 1 6.12
AAAAAAAAACAGAAAAA 1 6.12
AAAAAAAAAGAAAAAAA 1 6.12
AAAAAAAAAGAGCCTGC 1 6.12
AAAAAAAAATAGAAAAA 4 24.47

Total number of rows: 38745

Table truncated, full table size 958 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap