|
| Status |
Public on May 10, 2008 |
| Title |
Diff-Luminal |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
154531 |
| Tag Length |
17 |
| |
|
| Source name |
Differentiated luminal Cells of mammary tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: reduction mammoplasty sample
Gender: female
Age: 26 days
|
| Biomaterial provider |
Dr. Connie Eaves (ceaves@bccrc.ca), BC Cancer Research Centre
|
| Treatment protocol |
Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspensions and co-cultured with irradiated mouse fibroblasts for 3 days.
|
| Growth protocol |
Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
|
| |
|
| Description |
Highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal human mammary tissue were isolated and compared their transcriptomes obtained using PCR-LONG-SAGE protocol (Zhao, et al, Stem Cells, 2007)
|
| |
|
| Submission date |
May 08, 2008 |
| Last update date |
Nov 19, 2008 |
| Contact name |
BCGSC BC Cancer Agency |
| E-mail(s) |
support@mouseatlas.org
|
| Organization name |
Canada's Michael Smith Genome Sciences Centre
|
| Department |
Gene Expression
|
| Lab |
Marco Marra
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1485 |
| Series (2) |
| GSE11387 |
Transcript Analysis of Human Mammary Cell Subsets using SAGE |
| GSE11395 |
Transcript Analysis of Human Mammary Cell Subsets |
|
