|
Status |
Public on Feb 01, 2009 |
Title |
subcutaneous fat; KO; 4051964012_C |
Sample type |
RNA |
|
|
Source name |
KO
|
Organism |
Mus musculus |
Characteristics |
subcutaneous fat C57B6J mouse overnight starve 4 month old
|
Extracted molecule |
total RNA |
Extraction protocol |
Illumina protocol 3ug of total RNA was extracted from frozen tissue using QIAGEN Rneasy mini kit. This was submitted to the SRC Microarray Facility at the Institute for Molecular Bioscience at the University of Queensland for processing using Illuminar technology
|
Label |
biotin
|
Label protocol |
Illumina protocol
|
|
|
Hybridization protocol |
Illumina protocol
|
Scan protocol |
Illumina protocol
|
Description |
Growth hormone receptor was truncated at amino acid 569, amino acid 391, mutated within the Box1 region, or removed altogether (KO) and transgenic mice were created. At 4 months of age male mice were starved overnight and sacrificed.
|
Data processing |
The raw data were uploaded into GeneSpring GX for analysis
|
|
|
Submission date |
May 09, 2008 |
Last update date |
Nov 25, 2008 |
Contact name |
Johanna Barclay |
E-mail(s) |
j.barclay@imb.uq.edu.au
|
Organization name |
Institute for Molecular Bioscience
|
Lab |
Growth Hormone and Cytokine Signalling
|
Street address |
Building 80, University of Queensland
|
City |
ST Lucia |
State/province |
Queensland |
ZIP/Postal code |
4072 |
Country |
Australia |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE11396 |
Gene expression of transgenic knock-in mutant growth hormone receptor mice |
|