|
| Status |
Public on Mar 27, 2018 |
| Title |
M2 macrophages, control, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: M2 macrophages
polarization state: M2 like
|
| Treatment protocol |
Mature M2 macrophages were transfected with either mimic or inhibitor using the Lipofectamin RNAiMAX reagent according to manufacturer instructions.
|
| Growth protocol |
Blood was obtained from healthy donors. PBMCs from buffy coats were isolated. Subsequently, PBMCs underwent negative selection for CD14 Monocytes. Selected monocytes were then seeded in M2-activation medium . After 6 days additional boost of fresh media were added together with IL-4 (50ng/ml) . After 3 more days (day 9) media were replaced and cytokines were added as in day 6.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the miRNeasy Micro Kit (QIagen) according to manufactures protocol. RNA was quantified using a Nanodrop ND-1000 (Thermo Fischer Scientific) and Bioanalyser (Agilent).
|
| Label |
total RNA
|
| Label protocol |
Sense strand cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit.
|
| |
|
| Hybridization protocol |
Four replicates of each group were hybridized to Affymetrix mouse (human) Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
|
| Scan protocol |
Wash and stain were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
|
| Description |
Gene expression data from macrophages transfected with scramble control
|
| Data processing |
The data were analyzed with BRBarrayTools settings with RMA normalization and general background correction.
|
| |
|
| Submission date |
Dec 08, 2017 |
| Last update date |
Mar 27, 2018 |
| Contact name |
Ana I Robles |
| E-mail(s) |
Ana_Robles@nih.gov
|
| Phone |
301-496-1729
|
| Organization name |
National Cancer Institute/NIH
|
| Lab |
Laboratory of Human Carcinogenesis
|
| Street address |
37 Convent Dr 37/3060D
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE107870 |
Expression data from human macrophages treate with miR-1246 mimic/inhibitor |
|
