|
| Status |
Public on Dec 12, 2017 |
| Title |
mouse embryonic stem cell rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic stem cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: embryonic stem cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Gene Chip WT PLUS Reagent kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.3 mg of fragmented and labeled ss-cDNA were hybridized for 16 hr at 45℃ on GeneChip Clariom S array, mouse. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Gene chip Scanner 7G, affymetrix.
|
| Description |
mESC_rep1
|
| Data processing |
affymetrix-algorithm-name = sst-rma-gene-full
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Dec 11, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Toshiyuki Takehara |
| E-mail(s) |
takehara@med.kindai.ac.jp
|
| Organization name |
Kindai Universty Hospital
|
| Department |
Faculty of Medicine
|
| Lab |
Regenerative Medicine
|
| Street address |
377-2, Ohno-Higashi
|
| City |
Osaka-Sayama |
| State/province |
Osaka |
| ZIP/Postal code |
5898511 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE107889 |
Characterization of distinct states of mouse naïve and primed pluriopotency |
|
