|
| Status |
Public on Aug 22, 2008 |
| Title |
retina-control-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
retina, untreated
|
| Organism |
Gallus gallus |
| Characteristics |
Strain: White Leghorn
Gender: male
Age: 9 days
|
| Biomaterial provider |
A.C. Weiss GmbH & Co. KG, Kirchberg, Germany
|
| Treatment protocol |
One day prior to the experiment (age: 8 days), velcro rings were glued to the feathers around the eye under diethylether anaesthesia. At the age of 9 days, after 4-6 hours of light-onset, plus 6.9 diopter lenses were fitted to both eyes of the plus_lens treated group, control chicks received no lenses. 24 hours later, Chicks were killed by an overdose of diethylether, decapitated and the eyes were enucleated. Retinal samples were snap-frozen in liquid nitrogen and stored at -80°C.
|
| Growth protocol |
chicks were raised in groups under a 12-hr light/12-hr dark cycle with free access to food and water. Illumination was provided by light bulbs that produced an ambient illuminance of about 1000 lux on the cage floor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The retinae of both eyes were pooled and RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instruction. RNA was quantified and checked for quality with the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). The RNA integrity numbers (RIN) ranged from 9.2 to 9.5 (with 1 being the most degraded profile and 10 being the most intact).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 ug total RNA (see manufacturer´s website: Expression Analysis Technical Manual, One-Cycle Target Labeling Assay, Affymetrix).
|
| |
|
| Hybridization protocol |
see manufacturer's website
|
| Scan protocol |
see manufacturer's website
|
| Description |
Four samples each: Retina of both eyes of +6.9 diopter lens-treated chicks (plus_lens), Retina of both eyes of untreated control chicks (control)
|
| Data processing |
transformation algorithm: GC-RMA
|
| |
|
| Submission date |
May 14, 2008 |
| Last update date |
Aug 22, 2008 |
| Contact name |
Marita Pauline Feldkaemper |
| E-mail(s) |
marita.feldkaemper@uni-tuebingen.de
|
| Phone |
+49-7071-2987424
|
| Organization name |
University Eye Hospital Tuebingen, Institute for Ophthalmic Research
|
| Department |
Section of Neurobiology of the Eye
|
| Lab |
Prof. Dr. Frank Schaeffel
|
| Street address |
Calwerstrasse 7/1
|
| City |
Tuebingen |
| ZIP/Postal code |
72074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE11439 |
Retinal gene expression in chicks during imposed myopic defocus |
|
