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Sample GSM2886618 Query DataSets for GSM2886618
Status Public on Jun 19, 2018
Title COS-7_siTRA1
Sample type RNA
 
Source name COS-7_siTRA
Organism Chlorocebus sabaeus
Characteristics tissue source: kidney
cell line: COS-7
condition: knockdown for TRA2A (cond3)
Extracted molecule total RNA
Extraction protocol RNA was extracted following Qiagen protocol including DNAse treatment
Label biotin
Label protocol 100ng of total RNA from each sample, isolated from COS-7 cells under different experimental conditions was processed, labelled according to the Affymetrix GeneChip® Whole Transcript Plus protocol
 
Hybridization protocol Sample was hybridized to Affymetrix Human Clariom D array using a Affymetrix GeneChip Hybridization Oven 645
Scan protocol GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
Description siTRA1_(Clariom_D_Human).sst-rma-gene-full.chp Signal
Data processing The data were normalized using the SST-RMA algorithm and analyzed in the Transcriptome Analysis Console (TAC, Affymetrix)
The 'RMA_SST_ExonNormalization.txt' contains Exon log2 SST-RMA data
 
Submission date Dec 13, 2017
Last update date Oct 19, 2023
Contact name Júlia Perera-Bel
E-mail(s) mardata-bu@researchmar.net
Organization name Hospital del Mar Research Institute
Department MARData-BU
Street address Doctor Aiguader, 88
City Barcelona
ZIP/Postal code 08003
Country Spain
 
Platform ID GPL23126
Series (1)
GSE108007 The scaffolding role of RNA in ribonucleoprotein complexes: TRA2A recruitment in FXTAS disease

Data table header descriptions
ID_REF
VALUE Gene log2 SST-RMA data

Data table
ID_REF VALUE
TC0700010472.hg.1 11.4
TC0700009231.hg.1 11.9
TC0500009706.hg.1 14.89
TC1700011984.hg.1 9.97
TC2200007204.hg.1 11.65
TC0800011703.hg.1 4.2
TC1500006994.hg.1 11.71
TC0100009364.hg.1 14.36
TC1900007384.hg.1 10.57
TC0100013561.hg.1 10.53
TC0400008091.hg.1 8.14
TC2000007281.hg.1 6.13
TC0600008350.hg.1 12.72
TC0700009807.hg.1 12.46
TC0700006913.hg.1 13.85
TC0300013420.hg.1 13.04
TC1000006924.hg.1 12.11
TC0100011770.hg.1 12.39
TC0700009230.hg.1 9.59
TC1400009214.hg.1 10.78

Total number of rows: 135750

Table truncated, full table size 3049 Kbytes.




Supplementary file Size Download File type/resource
GSM2886618_siTRA1_Clariom_D_Human_.CEL.gz 22.9 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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