|
| Status |
Public on Sep 03, 2018 |
| Title |
crypt from CSN8fl/fl mice,biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
crypt from CSN8fl/fl mice
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57bl/6
age: 16 week
gender: female
genotype/variation: CSN8fl/fl
tissue/cell type: small intestinal crypts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from crypts was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
the GeneChip® WT PLUS Reagent kit protocol from Affymetrix
|
| |
|
| Hybridization protocol |
Affymetrix Mouse ClariomTM S arrays
|
| Scan protocol |
The arrays were processed following the manufacturer’s recommended wash and stain protocol on an Affymetrix FS-450 fluidics station and scanned on an Affymetrix GeneChip® 7G scanner using the Command Console 4.0
|
| Description |
Gene expression data from crypt
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.6 and transcripts were normalized on a gene level using RMA as a normalization and background correction method
|
| |
|
| Submission date |
Dec 15, 2017 |
| Last update date |
Sep 03, 2018 |
| Contact name |
Zhong-bin Deng |
| Organization name |
University of Louisville
|
| Department |
Medicine
|
| Street address |
505 s Hancock street
|
| City |
Louisville |
| ZIP/Postal code |
40202 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
|
