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Status |
Public on Sep 03, 2018 |
Title |
crypt from CSN8fl/fl mice,biological rep3 |
Sample type |
RNA |
|
|
Source name |
crypt from CSN8fl/fl mice
|
Organism |
Mus musculus |
Characteristics |
strain background: C57bl/6 age: 10 week gender: male genotype/variation: CSN8fl/fl tissue/cell type: small intestinal crypts
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from crypts was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol
|
Label |
biotin
|
Label protocol |
the GeneChip® WT PLUS Reagent kit protocol from Affymetrix
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|
|
Hybridization protocol |
Affymetrix Mouse ClariomTM S arrays
|
Scan protocol |
The arrays were processed following the manufacturer’s recommended wash and stain protocol on an Affymetrix FS-450 fluidics station and scanned on an Affymetrix GeneChip® 7G scanner using the Command Console 4.0
|
Description |
Gene expression data from crypt
|
Data processing |
The data were analyzed with Partek Genomics Suite 6.6 and transcripts were normalized on a gene level using RMA as a normalization and background correction method
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|
|
Submission date |
Dec 15, 2017 |
Last update date |
Sep 03, 2018 |
Contact name |
Zhong-bin Deng |
Organization name |
University of Louisville
|
Department |
Medicine
|
Street address |
505 s Hancock street
|
City |
Louisville |
ZIP/Postal code |
40202 |
Country |
USA |
|
|
Platform ID |
GPL23038 |
Series (1) |
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