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Sample GSM289390 Query DataSets for GSM289390
Status Public on May 19, 2008
Title rpL35 +/- MPNST_55
Sample type RNA
 
Source name Malignant peripheral nerve sheath tumors from fish heterozygous for inactivating mutation in ribosomal protein L35
Organism Danio rerio
Characteristics Late stage MPNST from zebrafish fish 18-24 months old
Treatment protocol Tumors were dissected from zebrafish anaesthetized with 0.03% MESAB
Growth protocol Zebrafish raised according to standard protocols
Extracted molecule total RNA
Extraction protocol Trizol according to the manufacturer's protocol
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Zebrafish Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 (A4 samples) or 450 (A5 and A7 samples).
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (A4 samples) or Affymetrix 7G scanner (A5 and A7 samples).
Description no additional information
Data processing Bioconductor Affy package and gcRMA
 
Submission date May 19, 2008
Last update date May 19, 2008
Contact name Charles Arthur Whittaker
E-mail(s) charliew@mit.edu
Organization name Koch Institute
Street address 77 Mass Ave 76-189
City Cambridge
State/province MA
ZIP/Postal code 02152
Country USA
 
Platform ID GPL1319
Series (1)
GSE11493 Comparison of transcripts in zebrafish tumors

Data table header descriptions
ID_REF
VALUE gcRMA-calculated, log2 expression values

Data table
ID_REF VALUE
Dr.1.1.S1_at 5.876159467
Dr.10.1.S1_at 1.704354783
Dr.10.4.S1_a_at 2.296382838
Dr.100.1.A1_at 2.726776054
Dr.1000.1.S1_at 11.76902434
Dr.10000.1.A1_at 1.509886016
Dr.10005.1.A1_at 8.290011675
Dr.10006.1.A1_at 9.791457082
Dr.10010.1.A1_at 2.611753133
Dr.10011.1.A1_at 7.425143144
Dr.10012.1.A1_at 4.273705285
Dr.10014.1.A1_at 1.815593216
Dr.10015.1.S1_at 3.099427122
Dr.10015.2.S1_a_at 3.350104641
Dr.10015.2.S1_at 2.224226019
Dr.10016.1.A1_at 7.255877127
Dr.10018.1.S1_at 2.180147373
Dr.1002.1.S1_at 8.39555314
Dr.10022.1.S1_at 6.125760411
Dr.10023.1.A1_at 1.889229669

Total number of rows: 15502

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM289390.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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