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Sample GSM289395 Query DataSets for GSM289395
Status Public on May 19, 2008
Title Seminoma-17
Sample type RNA
 
Source name Seminoma
Organism Danio rerio
Characteristics Seminoma from fish 36 months old
Treatment protocol Tumors were dissected from zebrafish anaesthetized with 0.03% MESAB
Growth protocol Zebrafish raised according to standard protocols
Extracted molecule total RNA
Extraction protocol Trizol according to the manufacturer's protocol
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Zebrafish Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 (A4 samples) or 450 (A5 and A7 samples).
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (A4 samples) or Affymetrix 7G scanner (A5 and A7 samples).
Description no additional information
Data processing Bioconductor Affy package and gcRMA
 
Submission date May 19, 2008
Last update date May 19, 2008
Contact name Charles Arthur Whittaker
E-mail(s) charliew@mit.edu
Organization name Koch Institute
Street address 77 Mass Ave 76-189
City Cambridge
State/province MA
ZIP/Postal code 02152
Country USA
 
Platform ID GPL1319
Series (1)
GSE11493 Comparison of transcripts in zebrafish tumors

Data table header descriptions
ID_REF
VALUE gcRMA-calculated, log2 expression values

Data table
ID_REF VALUE
Dr.1.1.S1_at 6.957514554
Dr.10.1.S1_at 1.577465796
Dr.10.4.S1_a_at 2.186187287
Dr.100.1.A1_at 3.634242838
Dr.1000.1.S1_at 10.07423828
Dr.10000.1.A1_at 1.373504035
Dr.10005.1.A1_at 8.890496074
Dr.10006.1.A1_at 9.157813184
Dr.10010.1.A1_at 2.49315214
Dr.10011.1.A1_at 8.256988445
Dr.10012.1.A1_at 4.213492293
Dr.10014.1.A1_at 1.696903102
Dr.10015.1.S1_at 2.758812587
Dr.10015.2.S1_a_at 2.809662245
Dr.10015.2.S1_at 1.986895183
Dr.10016.1.A1_at 7.538797232
Dr.10018.1.S1_at 1.942298665
Dr.1002.1.S1_at 9.188098735
Dr.10022.1.S1_at 5.940421399
Dr.10023.1.A1_at 1.752976185

Total number of rows: 15502

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM289395.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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