|
| Status |
Public on May 19, 2008 |
| Title |
rpS5 +/- MPNST |
| Sample type |
RNA |
| |
|
| Source name |
Malignant peripheral nerve sheath tumors from fish heterozygous for inactivating mutation in ribosomal protein S5
|
| Organism |
Danio rerio |
| Characteristics |
Late stage MPNST from zebrafish fish 18-24 months old
|
| Treatment protocol |
Tumors were dissected from zebrafish anaesthetized with 0.03% MESAB
|
| Growth protocol |
Zebrafish raised according to standard protocols
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol according to the manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Zebrafish Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 (A4 samples) or 450 (A5 and A7 samples).
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner (A4 samples) or Affymetrix 7G scanner (A5 and A7 samples).
|
| Description |
no additional information
|
| Data processing |
Bioconductor Affy package and gcRMA
|
| |
|
| Submission date |
May 19, 2008 |
| Last update date |
May 19, 2008 |
| Contact name |
Charles Arthur Whittaker |
| E-mail(s) |
charliew@mit.edu
|
| Organization name |
Koch Institute
|
| Street address |
77 Mass Ave 76-189
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02152 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE11493 |
Comparison of transcripts in zebrafish tumors |
|
