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Sample GSM289507 Query DataSets for GSM289507
Status Public on Oct 01, 2008
Title Celiac Disease Sample 2_CAP152832
Sample type RNA
 
Source name Total RNA from peripheral blood RNA.
Organism Homo sapiens
Characteristics disease state: Celiac Disease
tissue: Peripheral blood
Extracted molecule total RNA
Extraction protocol PAXgene Blood RNA isolation kit protocol (Qiagen, UK, 762174)
Label Cy3
Label protocol 2.5 ml of peripheral blood was collected into a PAXgene tube (Becton Dickinson, UK, 762165). PAXgene vials were chosen to prevent density gradient centrifugation, immortalization or in vitro cell culture artifacts changing mRNA profiles. PAXgene tubes were mixed gently and incubated at room temperature for two hours. After collection, tubes were frozen at -20 degrees Celsius for at least 24 hours followed by storage at -80 degrees Celsius. RNA was isolated using the PAXgene Blood RNA isolation kit (Qiagen, UK, 762174). RNA was quantified using the Nanodrop (Nanodrop Technologies, USA). Total RNA integrity was analyzed using an Agilent Bioanalyzer (Agilent Technologies, USA).
 
Hybridization protocol Complementary RNA was hybridized to Illumina HumanRef-8 v2 Whole Genome BeadChips
Scan protocol Illumina BeadArray Reader, data was handled through Illumina BeadStudio Gene Expression module v3
Description Celiac Disease Sample 2_CAP152832
Data processing Quantile-quantile normalization.
 
Submission date May 19, 2008
Last update date Mar 12, 2010
Contact name Graham Heap
E-mail(s) g.heap@qmul.ac.uk
Organization name QMUL
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AT
Country United Kingdom
 
Platform ID GPL6104
Series (1)
GSE11501 Primary human leucocyte RNA expression of unrelated celiac disease cases and unrelated healthy controls.

Data table header descriptions
ID_REF
VALUE Quantile-quantile normalized ln expression intensity.

Data table
ID_REF VALUE
ILMN_1809034 4.429874612081457
ILMN_1660305 4.780295117185187
ILMN_1792173 4.686761170098308
ILMN_1762337 4.100718168751599
ILMN_1736007 4.142481242454796
ILMN_1731507 4.038206947880369
ILMN_1745607 4.158138018289407
ILMN_1757454 4.341294298541581
ILMN_1735045 4.221543269250357
ILMN_1680754 4.288130815091185
ILMN_1659452 4.029117107310047
ILMN_1767388 4.452774077829228
ILMN_1673870 4.100047456440743
ILMN_1675204 4.220022619036982
ILMN_1755321 4.564214786755155
ILMN_1698554 4.572036891028918
ILMN_1805938 4.155600123096493
ILMN_1676336 4.614925863286133
ILMN_1752884 4.204597762412278
ILMN_1809959 4.265316417969439

Total number of rows: 18981

Table truncated, full table size 574 Kbytes.




Supplementary file Size Download File type/resource
GSM289507_2_CAP152832.txt.gz 176.6 Kb (ftp)(http) TXT
Processed data included within Sample table

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