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Sample GSM289519 Query DataSets for GSM289519
Status Public on Oct 01, 2008
Title Celiac Disease Sample 1_CAP153115
Sample type RNA
 
Source name Total RNA from peripheral blood RNA.
Organism Homo sapiens
Characteristics disease state: Celiac Disease
tissue: Peripheral blood
Extracted molecule total RNA
Extraction protocol PAXgene Blood RNA isolation kit protocol (Qiagen, UK, 762174)
Label Cy3
Label protocol 2.5 ml of peripheral blood was collected into a PAXgene tube (Becton Dickinson, UK, 762165). PAXgene vials were chosen to prevent density gradient centrifugation, immortalization or in vitro cell culture artifacts changing mRNA profiles. PAXgene tubes were mixed gently and incubated at room temperature for two hours. After collection, tubes were frozen at -20 degrees Celsius for at least 24 hours followed by storage at -80 degrees Celsius. RNA was isolated using the PAXgene Blood RNA isolation kit (Qiagen, UK, 762174). RNA was quantified using the Nanodrop (Nanodrop Technologies, USA). Total RNA integrity was analyzed using an Agilent Bioanalyzer (Agilent Technologies, USA).
 
Hybridization protocol Complementary RNA was hybridized to Illumina HumanRef-8 v2 Whole Genome BeadChips
Scan protocol Illumina BeadArray Reader, data was handled through Illumina BeadStudio Gene Expression module v3
Description Celiac Disease Sample 1_CAP153115
Data processing Quantile-quantile normalization.
 
Submission date May 19, 2008
Last update date Mar 12, 2010
Contact name Graham Heap
E-mail(s) g.heap@qmul.ac.uk
Organization name QMUL
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AT
Country United Kingdom
 
Platform ID GPL6104
Series (1)
GSE11501 Primary human leucocyte RNA expression of unrelated celiac disease cases and unrelated healthy controls.

Data table header descriptions
ID_REF
VALUE Quantile-quantile normalized ln expression intensity.

Data table
ID_REF VALUE
ILMN_1809034 4.412115744192928
ILMN_1660305 4.550998589327895
ILMN_1792173 4.567587564545468
ILMN_1762337 4.056589841295002
ILMN_1736007 4.104513654074196
ILMN_1731507 4.064196712889273
ILMN_1745607 4.2826239752126805
ILMN_1757454 4.329994507488188
ILMN_1735045 4.18253595282146
ILMN_1680754 4.333988349768501
ILMN_1659452 4.175141722521476
ILMN_1767388 4.364229722242025
ILMN_1673870 4.153483069263722
ILMN_1675204 4.384280976631031
ILMN_1755321 4.38887591810159
ILMN_1698554 4.761030399062096
ILMN_1805938 4.234413830374332
ILMN_1676336 4.666487635396409
ILMN_1752884 4.288251911246383
ILMN_1809959 4.350045824427291

Total number of rows: 18981

Table truncated, full table size 574 Kbytes.




Supplementary file Size Download File type/resource
GSM289519_1_CAP153115.txt.gz 175.9 Kb (ftp)(http) TXT
Processed data included within Sample table

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