|
| Status |
Public on Oct 01, 2008 |
| Title |
Celiac Disease Sample 3_CAP152834 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from peripheral blood RNA.
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Celiac Disease
tissue: Peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PAXgene Blood RNA isolation kit protocol (Qiagen, UK, 762174)
|
| Label |
Cy3
|
| Label protocol |
2.5 ml of peripheral blood was collected into a PAXgene tube (Becton Dickinson, UK, 762165). PAXgene vials were chosen to prevent density gradient centrifugation, immortalization or in vitro cell culture artifacts changing mRNA profiles. PAXgene tubes were mixed gently and incubated at room temperature for two hours. After collection, tubes were frozen at -20 degrees Celsius for at least 24 hours followed by storage at -80 degrees Celsius. RNA was isolated using the PAXgene Blood RNA isolation kit (Qiagen, UK, 762174). RNA was quantified using the Nanodrop (Nanodrop Technologies, USA). Total RNA integrity was analyzed using an Agilent Bioanalyzer (Agilent Technologies, USA).
|
| |
|
| Hybridization protocol |
Complementary RNA was hybridized to Illumina HumanRef-8 v2 Whole Genome BeadChips
|
| Scan protocol |
Illumina BeadArray Reader, data was handled through Illumina BeadStudio Gene Expression module v3
|
| Description |
Celiac Disease Sample 3_CAP152834
|
| Data processing |
Quantile-quantile normalization.
|
| |
|
| Submission date |
May 19, 2008 |
| Last update date |
Mar 12, 2010 |
| Contact name |
Graham Heap |
| E-mail(s) |
g.heap@qmul.ac.uk
|
| Organization name |
QMUL
|
| Street address |
4 Newark Street
|
| City |
London |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE11501 |
Primary human leucocyte RNA expression of unrelated celiac disease cases and unrelated healthy controls. |
|
