Cells were grown to saturation in YPD (YEP + 2% glucose) for 24 hours, diluted into YPA (YEP + 2% potassium acetate) at OD600= 0.3 and grown over night at 30 degree C. Cells were washed with sterilized water the next day and re-suspended in SPO medium (0.3% potassium acetate, pH = 7.0) at OD600= 1.9 at room temperature to induce sporulation. Benomyl [Methyl 1-(butylcarbamoyl)-2-benzimidazolecarbamate, Sigma-Aldrich; 30 mg/ml stock in DMSO] was dissolved to a final concentration of 0.4% in near-boiling SPO medium to avoid precipitation. The medium was then allowed to slowly cool to room temperature. 4 h after induction of sporulation cells were filtered and immediately re-suspended in medium containing Benomyl. The sample was taken 1 h after treatment with Benomyl. Keywords = benomyl sporulation saccharomyces microtubule spindle checkpoint
A novel response to microtubule perturbation in meiosis
Data table header descriptions
ID_REF
VALUE
Normalized by variance stabilization (vsn), summarized using median polish, only perfect match. Exact procedure, see: http://www.bioz.unibas.ch/primig/benomyl/
