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Sample GSM291228 Query DataSets for GSM291228
Status Public on Feb 19, 2009
Title GPR30-antisense Transfection, OHT Induction 1h, B
Sample type RNA
 
Source name SKBR3
Organism Homo sapiens
Characteristics Estrogen Receptor alpha and beta negative breast cancer cells
Treatment protocol SKBR3 cells were transfected in 10 cm plates at roughly 60% density with Fugene for 30 hours (3microl/microgram). Cells were induced for 1 h with respective treatment.
Growth protocol SKBR3 cells were grown in RPMI-1640 media supplemented with 10% FCS and L-Glutamine. Cells were switched to media without serum 24 h before transfection.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TriZOL.
Label biotin UTP
Label protocol As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip, Illumina TotalPrep RNA Amplification Kit from Ambion, Catalog #IL1791
 
Hybridization protocol As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip
Scan protocol As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip
Description Gene expression analyses were carried out to identify genes regulated by 17-beta estradiol (E2) and Hydroxytamoxifen (OHT) through GPR30 in SKBR3 cells.
Data processing Illumina Bead Studio, No background subtraction, No normalization
 
Submission date May 27, 2008
Last update date Feb 19, 2009
Contact name Deo Prakash Pandey
E-mail(s) Deo.Pandey@unige.ch
Phone 0041 22 3796811
Fax 0041 22 3796442
URL http://www.picard.ch
Organization name University of Geneva
Department Cell Biology
Street address 30 Quai Ernest-Ansmert
City Geneva
ZIP/Postal code 1211
Country Switzerland
 
Platform ID GPL6102
Series (1)
GSE11567 GPR30-mediated estrogen signaling in Estrogen Receptor alpha and beta negative SKBR3 breast cancer cells

Data table header descriptions
ID_REF
VALUE Rquantile normalization
Detection.1718911072_E

Data table
ID_REF VALUE Detection.1718911072_E
ILMN_1804663 80.79563937 0.86608
ILMN_1840887 71.27798579 0.25691
ILMN_1867201 70.06257831 0.17831
ILMN_1900605 76.05513173 0.63828
ILMN_1651799 654.4222667 1
ILMN_1818166 84.55108654 0.94323
ILMN_1782558 71.39424805 0.26638
ILMN_1827636 106.0681056 0.99563
ILMN_1821792 71.76068661 0.30058
ILMN_1833080 72.70127178 0.38355
ILMN_1895660 69.2796822 0.12591
ILMN_1850244 73.77493959 0.4607
ILMN_1812262 1001.316333 1
ILMN_1712803 419.2829295 1
ILMN_1842171 71.61107894 0.28384
ILMN_1873045 84.24409584 0.9425
ILMN_1774757 78.40892551 0.7722
ILMN_1905009 77.81807356 0.74454
ILMN_1867479 75.08994235 0.56769
ILMN_1875979 75.9551055 0.63464

Total number of rows: 48693

Table truncated, full table size 1515 Kbytes.




Supplementary file Size Download File type/resource
GSM291228.txt.gz 777.3 Kb (ftp)(http) TXT
Processed data included within Sample table

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